TY - JOUR
T1 - Inositol 1,4,5-trisphosphate receptors
T2 - Distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation
AU - Danoff, Sonye K.
AU - Ferris, Christopher D.
AU - Donath, Cornelia
AU - Fischer, Gabriela A.
AU - Munemitsu, Susan
AU - Ullrich, Axel
AU - Snyder, Solomon H.
AU - Ross, Christopher A.
PY - 1991/4/1
Y1 - 1991/4/1
N2 - We have identified two distinct transcripts of inositol 1,4,5-trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The shorter form (lacking the insert) predominates in fetal brain and peripheral tissues and appears to represent a nonneuronal receptor, whereas the longer form is found in adult brain and appears to be exclusively neuronal. The phosphorylation kinetics by cAMP-dependent protein kinase and the phosphopeptide maps differ for inositol 1,4,5-trisphosphate receptors purified from tissues predominantly expressing different forms of the transcript.
AB - We have identified two distinct transcripts of inositol 1,4,5-trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The shorter form (lacking the insert) predominates in fetal brain and peripheral tissues and appears to represent a nonneuronal receptor, whereas the longer form is found in adult brain and appears to be exclusively neuronal. The phosphorylation kinetics by cAMP-dependent protein kinase and the phosphopeptide maps differ for inositol 1,4,5-trisphosphate receptors purified from tissues predominantly expressing different forms of the transcript.
KW - Calcium
KW - Calmodulin-dependent protein kinase
KW - Diacylglycerol
KW - Inositol phospholipid
KW - Protein kinase C
KW - cAMP-dependent protein kinase
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U2 - 10.1073/pnas.88.7.2951
DO - 10.1073/pnas.88.7.2951
M3 - Article
C2 - 1849282
AN - SCOPUS:0025857174
SN - 0027-8424
VL - 88
SP - 2951
EP - 2955
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -