Abstract
• Aim: To investigate the effects of pEGFP-TGF-β1 plasmid DNA on proliferation and survival of human lens epithelial cell. • Methods: Immortalized human lens epithelial cell line (HLEC- B3) was transfected by pEGFP-TGF-β1 genes, respectively. Cell proliferation was analyzed by cell growth curve and MTT colorimetric assay, cell apoptosis and cell cycle of HLEC-B3 were examined by flowcytometry. • Results: HLEC-B3 cells were transfected successfully, pEGFP-TGF-β1 suppressed the growth rate, enhanced the cell apoptosis(21.0±1.7) % after 24 hours and (43.6±1.4)% after 48 hours transfection. The proportions of G1 phase cells were increased to (72.0±1.9)% after 24 hours and (74.7±2.2)% after 48 hours transfection, while the proportions of S phase cells were decreased to(20.5±2.2)% after 24 hours and (19.4± 1.4)% after 48 hours transfection. • Conclusion: pEGFP-TGF-(31 genes can successfully transfect HLEC-B3, inhibit proliferation and induce apoptosis of HLEC-B3.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 357-360 |
| Number of pages | 4 |
| Journal | International Eye Science |
| Volume | 7 |
| Issue number | 2 |
| State | Published - Apr 2007 |
| Externally published | Yes |
Keywords
- Apoptosis
- Lens epithelial cells
- TGF-β1 genes
- Transfection
ASJC Scopus subject areas
- Ophthalmology
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