Inhibition of migration and invasion in melanoma cells by β-escin via the ERK/NF-κB signaling pathway

Hyeong Seob Kwak, Hongyan An, Md Badrul Alam, Won Sik Choi, Sang Yong Lee, Sang Han Lee

Research output: Contribution to journalArticlepeer-review


β-Escin, a natural triterpene saponin was extracted from Aesculus hippocastanum seeds, which have been widely used to treat inflammation in traditional medicine. In an effort to study the possible anti-tumor effects of β-escin, we performed wound healing, invasion, and adhesion assays to examine the effects of β-escin on cell migration, invasion, and angiogenesis. Our results revealed that β-escin inhibits cell migration as well as motility in B16F10 and SK-MEL5 cells in a dose-dependent manner. RT-PCR and Western blot analysis showed that β-escin increased TIMP-1, -2 while significantly downregulated phosphorylated extracellular signal-regulated kinase (p-ERK) expression, and suppressing nuclear factor-kappa B (NF-κB) and inhibitor of nuclear factor-kappa B (IκB) expression. Overall, the data from the current study suggest that β-escin has the potential for inhibiting both metastatic and angiogenic activities, and are the earliest evidence for the involvement of the NF-κB/IκB signaling in β-escin-induced anti-tumor effects.

Original languageEnglish (US)
Pages (from-to)1606-1610
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Issue number10
StatePublished - 2018
Externally publishedYes


  • Cell migration
  • Extracellular signal-regulated kinase
  • Invasion
  • Nuclear factor-kappaB (NF-κB)
  • β-escin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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