Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor

I. M. Orme; S. K. Furney; P. S. Skinner; A. D. Roberts; P. J. Brennan; D. G. Russell; H. Shiratsuchi; J. J. Ellner; W. Y. Weiser

Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor

I. M. Orme, S. K. Furney, P. S. Skinner, A. D. Roberts, P. J. Brennan, D. G. Russell, H. Shiratsuchi, J. J. Ellner, W. Y. Weiser

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Infections caused by Mycobacterium avium, the most common form of disseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12- kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.

Original language	English (US)
Pages (from-to)	338-342
Number of pages	5
Journal	Infection and Immunity
Volume	61
Issue number	1
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Immunology

Cite this

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title = "Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor",

abstract = "Infections caused by Mycobacterium avium, the most common form of disseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12- kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.",

author = "Orme, {I. M.} and Furney, {S. K.} and Skinner, {P. S.} and Roberts, {A. D.} and Brennan, {P. J.} and Russell, {D. G.} and H. Shiratsuchi and Ellner, {J. J.} and Weiser, {W. Y.}",

year = "1993",

language = "English (US)",

volume = "61",

pages = "338--342",

journal = "Infection and Immunity",

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T1 - Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor

AU - Orme, I. M.

AU - Furney, S. K.

AU - Skinner, P. S.

AU - Roberts, A. D.

AU - Brennan, P. J.

AU - Russell, D. G.

AU - Shiratsuchi, H.

AU - Ellner, J. J.

AU - Weiser, W. Y.

PY - 1993

Y1 - 1993

N2 - Infections caused by Mycobacterium avium, the most common form of disseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12- kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.

AB - Infections caused by Mycobacterium avium, the most common form of disseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12- kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.

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M3 - Article

C2 - 8418058

AN - SCOPUS:0027454803

SN - 0019-9567

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IS - 1

ER -

Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor

Abstract

ASJC Scopus subject areas

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