Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)

G. H. Greeley; J. C. Thompson; J. Ishizuka; C. W. Cooper; M. A. Levine; S. U. Gorr; D. V. Cohn

Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)

G. H. Greeley, J. C. Thompson, J. Ishizuka, C. W. Cooper, M. A. Levine, S. U. Gorr, D. V. Cohn

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of graded doses (10^-10-10^-8 M) of highly purified bovine parathyroid secretory protein-I (SP-I; chromogranin-A) or synthetic porcine pancreastatin on glucose-stimulated insulin release in the perfused rat pancreas was examined. SP-I (10^-9 M) inhibited the first phase of glucose-stimulated insulin release, and 10^-8 M SP-I inhibited both the first and second phases of glucose-stimulated insulin release; 10^-10 M SP-I was inactive. In comparison, pancreastatin at 10^-10 M inhibited the first phase of insulin release, and at 10^-9 and 10^-8 M, pancreastatin inhibited both phases of insulin release. The inhibition by SP-I was achieved at concentrations that normally exist in the general circulation of man. These and other data suggest that circulating SP-I plays a physiological role in the regulation of insulin secretion.

Original language	English (US)
Pages (from-to)	1235-1238
Number of pages	4
Journal	Endocrinology
Volume	124
Issue number	3
State	Published - 1989

ASJC Scopus subject areas

Endocrinology
Endocrinology, Diabetes and Metabolism

Cite this

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title = "Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)",

abstract = "The effect of graded doses (10-10-10-8 M) of highly purified bovine parathyroid secretory protein-I (SP-I; chromogranin-A) or synthetic porcine pancreastatin on glucose-stimulated insulin release in the perfused rat pancreas was examined. SP-I (10-9 M) inhibited the first phase of glucose-stimulated insulin release, and 10-8 M SP-I inhibited both the first and second phases of glucose-stimulated insulin release; 10-10 M SP-I was inactive. In comparison, pancreastatin at 10-10 M inhibited the first phase of insulin release, and at 10-9 and 10-8 M, pancreastatin inhibited both phases of insulin release. The inhibition by SP-I was achieved at concentrations that normally exist in the general circulation of man. These and other data suggest that circulating SP-I plays a physiological role in the regulation of insulin secretion.",

author = "Greeley, {G. H.} and Thompson, {J. C.} and J. Ishizuka and Cooper, {C. W.} and Levine, {M. A.} and Gorr, {S. U.} and Cohn, {D. V.}",

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AU - Greeley, G. H.

AU - Thompson, J. C.

AU - Ishizuka, J.

AU - Cooper, C. W.

AU - Levine, M. A.

AU - Gorr, S. U.

AU - Cohn, D. V.

PY - 1989

Y1 - 1989

N2 - The effect of graded doses (10-10-10-8 M) of highly purified bovine parathyroid secretory protein-I (SP-I; chromogranin-A) or synthetic porcine pancreastatin on glucose-stimulated insulin release in the perfused rat pancreas was examined. SP-I (10-9 M) inhibited the first phase of glucose-stimulated insulin release, and 10-8 M SP-I inhibited both the first and second phases of glucose-stimulated insulin release; 10-10 M SP-I was inactive. In comparison, pancreastatin at 10-10 M inhibited the first phase of insulin release, and at 10-9 and 10-8 M, pancreastatin inhibited both phases of insulin release. The inhibition by SP-I was achieved at concentrations that normally exist in the general circulation of man. These and other data suggest that circulating SP-I plays a physiological role in the regulation of insulin secretion.

AB - The effect of graded doses (10-10-10-8 M) of highly purified bovine parathyroid secretory protein-I (SP-I; chromogranin-A) or synthetic porcine pancreastatin on glucose-stimulated insulin release in the perfused rat pancreas was examined. SP-I (10-9 M) inhibited the first phase of glucose-stimulated insulin release, and 10-8 M SP-I inhibited both the first and second phases of glucose-stimulated insulin release; 10-10 M SP-I was inactive. In comparison, pancreastatin at 10-10 M inhibited the first phase of insulin release, and at 10-9 and 10-8 M, pancreastatin inhibited both phases of insulin release. The inhibition by SP-I was achieved at concentrations that normally exist in the general circulation of man. These and other data suggest that circulating SP-I plays a physiological role in the regulation of insulin secretion.

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Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)

Abstract

ASJC Scopus subject areas

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