Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation

Ding Xie Liu; Chang Cai Wang

Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation

Ding Xie Liu, Chang Cai Wang

Research output: Contribution to journal › Article › peer-review

Abstract

The triplex formation between a 21nt oligodeoxyribonucleotide G₃TG₂TGT₂G₅TG₂TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG₂TG₅T₂GTG₂TG₃), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.

Original language	English (US)
Pages (from-to)	265-266
Number of pages	2
Journal	Progress in Biochemistry and Biophysics
Volume	25
Issue number	3
State	Published - 1998
Externally published	Yes

Keywords

Antigene strategy
DNA binding protein
Hepatitis B virus
Oligodeoxyribonucleotide
Triplex DNA

ASJC Scopus subject areas

Biochemistry
Biophysics

Cite this

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title = "Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation",

abstract = "The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.",

keywords = "Antigene strategy, DNA binding protein, Hepatitis B virus, Oligodeoxyribonucleotide, Triplex DNA",

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year = "1998",

language = "English (US)",

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pages = "265--266",

journal = "Progress in Biochemistry and Biophysics",

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T1 - Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation

AU - Liu, Ding Xie

AU - Wang, Chang Cai

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Y1 - 1998

N2 - The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.

AB - The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.

KW - Antigene strategy

KW - DNA binding protein

KW - Hepatitis B virus

KW - Oligodeoxyribonucleotide

KW - Triplex DNA

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Inhibition of DNA binding protein binding to the core promoter of hepatitis B virus by triplex-formation

Abstract

Keywords

ASJC Scopus subject areas

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