Abstract
The triplex formation between a 21nt oligodeoxyribonucleotide G3TG2TGT2G5TG2TGT (CP1) and core promoter (Cp) fragment of hepatitis B virus (HBV) had a good specificity and stability, which was demonstrated by electrophoretic mobility shift analysis and DNase I footprinting experiment. Gel retardation assay showed CP1 could inhibit a specific cellular factor binding to Cp fragment in rat liver nuclear extracts. No inhibition of factor binding was observed by oligodeoxyribonucleotide CP3 (TGTG2TG5T2GTG2TG3), which could not form triplex with Cp fragment. These results indicate that specific repression of gene transcription of HBV DNA may be possible by triplex-formation.
Original language | English (US) |
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Pages (from-to) | 265-266 |
Number of pages | 2 |
Journal | Progress in Biochemistry and Biophysics |
Volume | 25 |
Issue number | 3 |
State | Published - 1998 |
Externally published | Yes |
Keywords
- Antigene strategy
- DNA binding protein
- Hepatitis B virus
- Oligodeoxyribonucleotide
- Triplex DNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics