@article{97fd835764524b099a7b8492a4d67f8f,
title = "Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates",
abstract = "Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, transmission studies rely on live isolates from non-human primates or endemic regions. Here, we demonstrate P. vivax gametocytes from both India and Brazil are stable during cryopreservation. Importantly, cryopreserved gametocytes from Brazil were capable of infecting three anopheline mosquito species in feedings done in the United States. These findings create new opportunities for transmission studies in diverse locales.",
keywords = "Anopheles, Cryopreservation, Gametocyte, Liver, Malaria, Plasmodium vivax, Sporozoite, Transmission",
author = "Kathryn Shaw-Saliba and David Clarke and Santos, {Jorge M.} and Menezes, {Maria Jos{\'e}} and Caeul Lim and Anjali Mascarenhas and Laura Chery and Edwin Gomes and Sandra March and Bhatia, {Sangeeta N.} and Rathod, {Pradipsinh K.} and Ferreira, {Marcelo U.} and Flaminia Catteruccia and Duraisingh, {Manoj T.}",
note = "Funding Information: The authors would like to acknowledge all of the patients and their families in Goa, India and Acrel{\^a}ndia, Brazil who gave consent to participate in the study. The authors gratefully thank the staff at Goa Medical College and the Malaria Evolution in South Asia (MESA) study. The authors also gratefully thank the researchers and staff at the University of S{\~a}o Paulo, Brazil who supported the Acrel{\^a}ndia Project. The authors were supported by a Bill and Melinda Gates, USA grant (OPP1023594, MTD). The Programme Project on MESA is supported by a United States National Institutes of Health/National Institute of Allergy and Infectious Disease, USA ICEMR (NIH/NIAID) Programme U19 AI089688 (PKR). The Brazilian project is supported by a NIH/NIAID ICEMR Programme U19 AI089681 (Joseph M. Vinetz) and the Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo, Brazil (FAPESP, 2009/52729-9 to MUF). MUF is supported by a senior researcher scholarship from the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) of Brazil. The Broad Institute BN10 provided support for KSS, DC, JMS. We also appreciate support from NIH grants R01 - AI104956 and R21- AI117313 to FC. Publisher Copyright: {\textcopyright} 2016 Australian Society for Parasitology",
year = "2016",
month = oct,
day = "1",
doi = "10.1016/j.ijpara.2016.06.003",
language = "English (US)",
volume = "46",
pages = "679--683",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "11",
}