Induction of vacuolar Ca2+-ATPase and H+/Ca2+ exchange activity in yeast mutants lacking Pmr1, the Golgi Ca2+-ATPase

Valerie Marchi; Alexander Sorin; Ying Wei; Rajini Rao

doi:10.1016/S0014-5793(99)00803-0

Induction of vacuolar Ca²⁺-ATPase and H⁺/Ca²⁺ exchange activity in yeast mutants lacking Pmr1, the Golgi Ca²⁺-ATPase

Valerie Marchi, Alexander Sorin, Ying Wei, Rajini Rao

School of Medicine

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

We have analyzed Ca²⁺ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca²⁺-ATPase, Pmr1, is the major Ca²⁺ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca²⁺-ATPase, Pmc1. In addition, H⁺/Ca²⁺ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	181-186
Number of pages	6
Journal	FEBS Letters
Volume	454
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00803-0
State	Published - Jul 9 1999

Keywords

Ca-ATPase
Calcium homeostasis
H/Ca exchanger
PMC1 gene
PMR1 gene
Saccharomyces cerevisiae

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(99)00803-0

Cite this

@article{e61917683250445eb8b9bead40362457,

title = "Induction of vacuolar Ca2+-ATPase and H+/Ca2+ exchange activity in yeast mutants lacking Pmr1, the Golgi Ca2+-ATPase",

abstract = "We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Ca-ATPase, Calcium homeostasis, H/Ca exchanger, PMC1 gene, PMR1 gene, Saccharomyces cerevisiae",

author = "Valerie Marchi and Alexander Sorin and Ying Wei and Rajini Rao",

note = "Funding Information: Acknowledgements: This work was supported by Grants from the American Cancer Society (IRG11-33 and JFRA 538), the American Heart Association (Grant-in-Aid 95012290) and the National Institutes of Health (GM52414) to RR and a Provost Award for Undergraduate Research by the The Johns Hopkins University to VM. We thank Kyle Cunningham for generously sharing yeast strains and plasmids and Zachary Skelding, who contributed to a portion of this study.",

year = "1999",

month = jul,

day = "9",

doi = "10.1016/S0014-5793(99)00803-0",

language = "English (US)",

volume = "454",

pages = "181--186",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Induction of vacuolar Ca2+-ATPase and H+/Ca2+ exchange activity in yeast mutants lacking Pmr1, the Golgi Ca2+-ATPase

AU - Marchi, Valerie

AU - Sorin, Alexander

AU - Wei, Ying

AU - Rao, Rajini

N1 - Funding Information: Acknowledgements: This work was supported by Grants from the American Cancer Society (IRG11-33 and JFRA 538), the American Heart Association (Grant-in-Aid 95012290) and the National Institutes of Health (GM52414) to RR and a Provost Award for Undergraduate Research by the The Johns Hopkins University to VM. We thank Kyle Cunningham for generously sharing yeast strains and plasmids and Zachary Skelding, who contributed to a portion of this study.

PY - 1999/7/9

Y1 - 1999/7/9

N2 - We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Ca-ATPase

KW - Calcium homeostasis

KW - H/Ca exchanger

KW - PMC1 gene

KW - PMR1 gene

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=0032587162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032587162&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)00803-0

DO - 10.1016/S0014-5793(99)00803-0

M3 - Article

C2 - 10431803

AN - SCOPUS:0032587162

SN - 0014-5793

VL - 454

SP - 181

EP - 186

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -