Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Ming Qiao; Mundrigi Ashok; Kristen A. Bernard; Gustavo Palacios; Z. Hong Zhou; W. Ian Lipkin; T. Jake Liang

doi:10.1086/425933

Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Ming Qiao, Mundrigi Ashok, Kristen A. Bernard, Gustavo Palacios, Z. Hong Zhou, W. Ian Lipkin, T. Jake Liang

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

Original language	English (US)
Pages (from-to)	2104-2108
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	190
Issue number	12
DOIs	https://doi.org/10.1086/425933
State	Published - Dec 15 2004
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Immunology

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10.1086/425933

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title = "Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells",

abstract = "No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.",

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AU - Qiao, Ming

AU - Ashok, Mundrigi

AU - Bernard, Kristen A.

AU - Palacios, Gustavo

AU - Zhou, Z. Hong

AU - Lipkin, W. Ian

AU - Liang, T. Jake

PY - 2004/12/15

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AB - No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

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Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Abstract

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