Induction of spinal cord neuronal nitric oxide synthase (NOS) after formalin injection in the rat hind paw

H. H.D. Lam; D. F. Hanley; B. D. Trapp; S. Saito; S. Raja; T. M. Dawson; H. Yamaguchi

doi:10.1016/0304-3940(96)12702-6

Induction of spinal cord neuronal nitric oxide synthase (NOS) after formalin injection in the rat hind paw

H. H.D. Lam, D. F. Hanley, B. D. Trapp, S. Saito, S. Raja, T. M. Dawson, H. Yamaguchi

School of Medicine

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

The nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester has been found to exhibit antinociceptive activity in a rat model of pain [Kitto, K.F. et al., Neurosci. Lett. 148 (1992) 1-5; Lee, J.H. et al., NeuroReport, 3 (1992) 841-844; Moore, P.K. et al., Br. J. Pharmacol., 102 (1991) 198-202]. We investigated the hypothesis that hind paw injection of formalin increases the number of dorsal horn neuronal nitric oxide synthase (nNOS) containing neurons. Results showed a bilateral increase in the number of nNOS-positive neurons at the L4-5 dorsal horn area following formalin injection. The increase was always greater on the ipsilateral side compared to the contralateral side. This upregulation of nNOS following formalin stimulation of the hind paw suggested that nitric oxide (NO) may play a role in the central mechanism of hyperalgesia that follows peripheral inflammation.

Original language	English (US)
Pages (from-to)	201-204
Number of pages	4
Journal	Neuroscience Letters
Volume	210
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12702-6
State	Published - Jun 7 1996

Keywords

Induction
Injury
Nitric oxide synthase
Pain
Spinal cord

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(96)12702-6

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title = "Induction of spinal cord neuronal nitric oxide synthase (NOS) after formalin injection in the rat hind paw",

abstract = "The nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester has been found to exhibit antinociceptive activity in a rat model of pain [Kitto, K.F. et al., Neurosci. Lett. 148 (1992) 1-5; Lee, J.H. et al., NeuroReport, 3 (1992) 841-844; Moore, P.K. et al., Br. J. Pharmacol., 102 (1991) 198-202]. We investigated the hypothesis that hind paw injection of formalin increases the number of dorsal horn neuronal nitric oxide synthase (nNOS) containing neurons. Results showed a bilateral increase in the number of nNOS-positive neurons at the L4-5 dorsal horn area following formalin injection. The increase was always greater on the ipsilateral side compared to the contralateral side. This upregulation of nNOS following formalin stimulation of the hind paw suggested that nitric oxide (NO) may play a role in the central mechanism of hyperalgesia that follows peripheral inflammation.",

keywords = "Induction, Injury, Nitric oxide synthase, Pain, Spinal cord",

author = "Lam, {H. H.D.} and Hanley, {D. F.} and Trapp, {B. D.} and S. Saito and S. Raja and Dawson, {T. M.} and H. Yamaguchi",

year = "1996",

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AU - Hanley, D. F.

AU - Trapp, B. D.

AU - Saito, S.

AU - Raja, S.

AU - Dawson, T. M.

AU - Yamaguchi, H.

PY - 1996/6/7

Y1 - 1996/6/7

N2 - The nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester has been found to exhibit antinociceptive activity in a rat model of pain [Kitto, K.F. et al., Neurosci. Lett. 148 (1992) 1-5; Lee, J.H. et al., NeuroReport, 3 (1992) 841-844; Moore, P.K. et al., Br. J. Pharmacol., 102 (1991) 198-202]. We investigated the hypothesis that hind paw injection of formalin increases the number of dorsal horn neuronal nitric oxide synthase (nNOS) containing neurons. Results showed a bilateral increase in the number of nNOS-positive neurons at the L4-5 dorsal horn area following formalin injection. The increase was always greater on the ipsilateral side compared to the contralateral side. This upregulation of nNOS following formalin stimulation of the hind paw suggested that nitric oxide (NO) may play a role in the central mechanism of hyperalgesia that follows peripheral inflammation.

AB - The nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester has been found to exhibit antinociceptive activity in a rat model of pain [Kitto, K.F. et al., Neurosci. Lett. 148 (1992) 1-5; Lee, J.H. et al., NeuroReport, 3 (1992) 841-844; Moore, P.K. et al., Br. J. Pharmacol., 102 (1991) 198-202]. We investigated the hypothesis that hind paw injection of formalin increases the number of dorsal horn neuronal nitric oxide synthase (nNOS) containing neurons. Results showed a bilateral increase in the number of nNOS-positive neurons at the L4-5 dorsal horn area following formalin injection. The increase was always greater on the ipsilateral side compared to the contralateral side. This upregulation of nNOS following formalin stimulation of the hind paw suggested that nitric oxide (NO) may play a role in the central mechanism of hyperalgesia that follows peripheral inflammation.

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KW - Injury

KW - Nitric oxide synthase

KW - Pain

KW - Spinal cord

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Induction of spinal cord neuronal nitric oxide synthase (NOS) after formalin injection in the rat hind paw

Abstract

Keywords

ASJC Scopus subject areas

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