Induction of Morphologically Abnormal Sperm in Rats Exposed to O-Xylene

W. J. Washington, R. C. Murthy, A. Doye, K. Eugene, D. Brown, I. Bradley

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalArchives of Andrology
Issue number3
StatePublished - Jan 1983
Externally publishedYes


  • Intraperitoneal injection
  • Mutagenicity
  • O-Xylene
  • Sperm abnormalities

ASJC Scopus subject areas

  • Endocrinology


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