TY - JOUR
T1 - Induction of immunodeficiency virus-specific immune responses in rhesus monkeys following gene gun-mediated DNA vaccination
AU - Fuller, Deborah Heydenburg
AU - Murphey-Corb, Michael
AU - Clements, Janice
AU - Barnett, Susan
AU - Haynes, Joel R.
PY - 1996/6
Y1 - 1996/6
N2 - The Accell® gene delivery system (gene gun) was used to deliver gold particles coated with HIV-1LAI and SIVmac239 expression constructs into the epidermis of rhesus macaques, resulting in the elicitation of env- and gag-specific humoral responses. One microgram of vector DNA per dose was sufficient to induce immune responses in monkeys using SIVmac239 gp160 and gp120 vectors driven by the CMV-intron A promoter. Several parameters, including the identity of the vector, the length of the rest period between immunizations, the number of immunizations, and the amount of DNA per immunization, are all important in designing an optimal DNA immunization regimen. In addition, gene gun-based DNA immunization using low efficiency expression vectors is an effective means of priming for the induction of vigorous antibody responses in macaques following boosting with recombinant subunits.
AB - The Accell® gene delivery system (gene gun) was used to deliver gold particles coated with HIV-1LAI and SIVmac239 expression constructs into the epidermis of rhesus macaques, resulting in the elicitation of env- and gag-specific humoral responses. One microgram of vector DNA per dose was sufficient to induce immune responses in monkeys using SIVmac239 gp160 and gp120 vectors driven by the CMV-intron A promoter. Several parameters, including the identity of the vector, the length of the rest period between immunizations, the number of immunizations, and the amount of DNA per immunization, are all important in designing an optimal DNA immunization regimen. In addition, gene gun-based DNA immunization using low efficiency expression vectors is an effective means of priming for the induction of vigorous antibody responses in macaques following boosting with recombinant subunits.
KW - HIV
KW - Nucleic acid vaccines
KW - Recombinant subunits
KW - SIV
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U2 - 10.1111/j.1600-0684.1996.tb00021.x
DO - 10.1111/j.1600-0684.1996.tb00021.x
M3 - Article
C2 - 8892045
AN - SCOPUS:0030155570
SN - 0047-2565
VL - 25
SP - 236
EP - 241
JO - Journal of medical primatology
JF - Journal of medical primatology
IS - 3
ER -