Induction of guinea pig antibody responses in vitro

P. L. Cohen, J. J. Ellner, E. M. Shevach

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8 Scopus citations


Guinea pig spleen cells cultured together with peritoneal exudate lymphocytes (PEL) were found to generate large numbers of antibody forming cells (AFC) in vitro in response to hapten protein antigens. Neither cell type cultured alone yielded appreciable responses. Strain 13 or F1 (Strain 2 x strain 13) lymphocytes, but not those from strain 2 animals, are able to respond to the genetically controlled antigen, DNP guinea pig albumin (DNP-GPA). Antisera directed against responder (strain 13) parent Ia antigens selectively blocked the generation of AFC by F1 (strain 2 x strain 13) spleen PEL mixtures in response to DNP-GPA. Both allogeneic (strain 2) and syngeneic macrophages functioned equally well in presentation of DNP-GPA to strain 13 lymphocytes.

Original languageEnglish (US)
Pages (from-to)2034-2039
Number of pages6
JournalJournal of Immunology
Issue number6
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Immunology


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