Induction of CRMP-4 in striatum of adult rat after transient brain ischemia

Peng Chong Liu, Zeng Jin Yang, Mei Hong Qiu, Ling Mei Zhang, Feng Yan Sun

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


AIM: To study the expression of collapsing response mediated protein-4 (CRMP-4) and nestin in the ischemic adult rat brain following transient brain ischemia. METHODS: Brain ischemia was induced by transient left middle cerebral artery occlusion (MCAO) for 60 min in adult rats. The expression of CRMP-4, nestin and bromodeoxyuridine (BrdU) was analyzed by immunohistochemical method. The co-localization of CRMP-4 and nestin or BrdU was analyzed by double staining combined with confocal laser scanning microscopy. RESULTS: CRMP-4, a marker of immature neuron, could be expressed in the ipsilateral striatum and cerebral cortex at 1st and 2nd week after the ischemia-reperfusion; nestin, a marker of neural stem cell, occurred in above regions from several hours to 2 weeks. CRMP-4 costained with nestin and with BrdU incorporation. CONCLUSION: Neural stem cells may present in the striatum and cerebral cortex of adult rat and can be triggered to differentiate into newborn neuron there by ischemic brain trauma.

Original languageEnglish (US)
Pages (from-to)1205-1211
Number of pages7
JournalActa Pharmacologica Sinica
Issue number12
StatePublished - Dec 2003
Externally publishedYes


  • Brain ischemia
  • Bromodeoxyuridine
  • CRMP-4 protein
  • Corpus striatum
  • Intermediate filament proteins
  • Neurons
  • Regeneration

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)


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