TY - JOUR
T1 - Induction of collagen by estradiol
T2 - Difference between sun-protected and photodamaged human skin in vivo
AU - Rittié, Laure
AU - Kang, Sewon
AU - Voorhees, John J.
AU - Fisher, Gary J.
PY - 2008/9
Y1 - 2008/9
N2 - Objective: To evaluate the effectiveness of topical estradiol in stimulating collagen I and III production in naturally aged and photoaged human skin of postmeno-pausal women and age-matched men. Design: Vehicle-controlled treatment followed by biochemical and immunohistochemical analyses of skin biopsy specimens. Setting: Academic referral center. Participants: Seventy healthy volunteers (40 postmeno-pausal women with a mean age of 75 years, and 30 men with a mean age of 75 years) with photodamaged skin. Interventions: Topical application of estradiol, 0.01%, 0.1%, 1%, or 2.5% or vehicle on aged or photoaged skin, with biopsy specimens taken after last treatment. Main Outcome Measures: De novo synthesis of collagen by quantitative polymerase chain reaction, immu-nohistochemistry, and enzyme-linked immunosorbent assay. Results: Topical estradiol increased procollagen I and III messenger RNA and collagen I protein levels in sun-protected aged hip skin in postmenopausal women and, to a lesser extent, in age-matched men. Surprisingly, no significant changes in production were observed in women or men after 2-week estradiol treatment of photoaged forearm or face skin, despite similar expression of estrogen receptors (ER-α, ER-β, and GPR30) in aged and photoaged skin. Estradiol treatment induced the estrogen-responsive gene GREBI, indicating that penetration of topical estradiol and genomic response to estrogen were similar in the 3 anatomic sites. Conclusions: Two-week topical estradiol treatment stimulates collagen production in sun-protected hip skin, but not in photoaged forearm or face skin, in postmenopausal women and aged-matched men. These findings suggest that menopause-associated estrogen decline is involved in reduced collagen production in sun-protected skin. Interestingly, alterations induced by long-term sun exposure hinder the ability of topical 2-week estradiol to stimulate collagen production in aged skin.
AB - Objective: To evaluate the effectiveness of topical estradiol in stimulating collagen I and III production in naturally aged and photoaged human skin of postmeno-pausal women and age-matched men. Design: Vehicle-controlled treatment followed by biochemical and immunohistochemical analyses of skin biopsy specimens. Setting: Academic referral center. Participants: Seventy healthy volunteers (40 postmeno-pausal women with a mean age of 75 years, and 30 men with a mean age of 75 years) with photodamaged skin. Interventions: Topical application of estradiol, 0.01%, 0.1%, 1%, or 2.5% or vehicle on aged or photoaged skin, with biopsy specimens taken after last treatment. Main Outcome Measures: De novo synthesis of collagen by quantitative polymerase chain reaction, immu-nohistochemistry, and enzyme-linked immunosorbent assay. Results: Topical estradiol increased procollagen I and III messenger RNA and collagen I protein levels in sun-protected aged hip skin in postmenopausal women and, to a lesser extent, in age-matched men. Surprisingly, no significant changes in production were observed in women or men after 2-week estradiol treatment of photoaged forearm or face skin, despite similar expression of estrogen receptors (ER-α, ER-β, and GPR30) in aged and photoaged skin. Estradiol treatment induced the estrogen-responsive gene GREBI, indicating that penetration of topical estradiol and genomic response to estrogen were similar in the 3 anatomic sites. Conclusions: Two-week topical estradiol treatment stimulates collagen production in sun-protected hip skin, but not in photoaged forearm or face skin, in postmenopausal women and aged-matched men. These findings suggest that menopause-associated estrogen decline is involved in reduced collagen production in sun-protected skin. Interestingly, alterations induced by long-term sun exposure hinder the ability of topical 2-week estradiol to stimulate collagen production in aged skin.
UR - http://www.scopus.com/inward/record.url?scp=51949096280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51949096280&partnerID=8YFLogxK
U2 - 10.1001/archderm.144.9.1129
DO - 10.1001/archderm.144.9.1129
M3 - Article
C2 - 18794456
AN - SCOPUS:51949096280
SN - 0003-987X
VL - 144
SP - 1129
EP - 1140
JO - Archives of Dermatology
JF - Archives of Dermatology
IS - 9
ER -