Abstract
Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.
Original language | English (US) |
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Pages (from-to) | 175-182 |
Number of pages | 8 |
Journal | International Journal of Developmental Neuroscience |
Volume | 15 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1997 |
Keywords
- Genes
- PC12
- Pb
- fos
ASJC Scopus subject areas
- Developmental Neuroscience
- Developmental Biology