Induction of c-foS mRNA by lead in PC12 cells

K. Kim; M. Annadata; G. W. Goldstein; J. P. Bressler

doi:10.1016/S0736-5748(96)00085-8

Induction of c-foS mRNA by lead in PC12 cells

K. Kim, M. Annadata, G. W. Goldstein, J. P. Bressler

Kennedy Krieger Institute

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb²⁺ has the potential to deregulate the expression of other genes.

Original language	English (US)
Pages (from-to)	175-182
Number of pages	8
Journal	International Journal of Developmental Neuroscience
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/S0736-5748(96)00085-8
State	Published - Apr 1997

Keywords

Genes
PC12
Pb
fos

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/S0736-5748(96)00085-8

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title = "Induction of c-foS mRNA by lead in PC12 cells",

abstract = "Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.",

keywords = "Genes, PC12, Pb, fos",

author = "K. Kim and M. Annadata and Goldstein, {G. W.} and Bressler, {J. P.}",

note = "Funding Information: Acknowledgments--The research in authors laboratory is supported by the following grants: N1EHS #ES02380-15; and NIEHS Center Grant #03819. The authors would like to thank Dr Pamela Talalay and Ms Angela T. Williams for their help in preparing this manuscript. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

month = apr,

doi = "10.1016/S0736-5748(96)00085-8",

language = "English (US)",

volume = "15",

pages = "175--182",

journal = "International Journal of Developmental Neuroscience",

issn = "0736-5748",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Induction of c-foS mRNA by lead in PC12 cells

AU - Kim, K.

AU - Annadata, M.

AU - Goldstein, G. W.

AU - Bressler, J. P.

N1 - Funding Information: Acknowledgments--The research in authors laboratory is supported by the following grants: N1EHS #ES02380-15; and NIEHS Center Grant #03819. The authors would like to thank Dr Pamela Talalay and Ms Angela T. Williams for their help in preparing this manuscript. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1997/4

Y1 - 1997/4

N2 - Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.

AB - Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.

KW - Genes

KW - PC12

KW - Pb

KW - fos

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U2 - 10.1016/S0736-5748(96)00085-8

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M3 - Article

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AN - SCOPUS:0030982114

SN - 0736-5748

VL - 15

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EP - 182

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

IS - 2

ER -

Induction of c-foS mRNA by lead in PC12 cells

Abstract

Keywords

ASJC Scopus subject areas

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