TY - JOUR
T1 - Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape
AU - Sodja, Ann
AU - Fujioka, Hisashi
AU - Lemos, Francisco J.A.
AU - Donnelly-Doman, Marilyn
AU - Jacobs-Lorena, Marcelo
N1 - Funding Information:
A.S. is indebted to V. Hari for use of some of the reagents and equipment and to T.J. Hadden and R.A. Thomas for helpful comments on the manuscript. F.J.A.L. was a fellow of the Brazilian National Research Council (CNPq). This work was supported by grants from the National Institutes of Health to M.J.-L.
PY - 2007/8
Y1 - 2007/8
N2 - Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.
AB - Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.
KW - Actin
KW - Anopheles gambiae
KW - Blood feeding
KW - Microvilli
KW - Midgut
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U2 - 10.1016/j.jinsphys.2007.03.017
DO - 10.1016/j.jinsphys.2007.03.017
M3 - Article
C2 - 17537455
AN - SCOPUS:34547699193
SN - 0022-1910
VL - 53
SP - 833
EP - 839
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
IS - 8
ER -