Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells

H. J. Broxterman; H. M. Pinedo; C. M. Kuiper; L. C M Kaptein; G. J. Schuurhuis; J. Lankelma

Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells

H. J. Broxterman, H. M. Pinedo, C. M. Kuiper, L. C M Kaptein, G. J. Schuurhuis, J. Lankelma

Research output: Contribution to journal › Article › peer-review

Abstract

A marked increase in cellular ATP consumption was induced by verapamil in the multidrug-resistant (MDR) cell line 2780(AD), but not in the drug-sensitive parental cell line A2780. A group of structurally unrelated drugs in concentrations known to reverse MDR, but not the verapamil analog tiapamil, a weak modulator of MDR, had similar effects. This effect was saturated at verapamil concentrations of about 1 μM. These data demonstrate that verapamil concentrations in MDR cells are maintained at a low level at the expense of ATP hydrolysis, and provide a first indication of the amount of metabolic energy used in this process.

Original language	English (US)
Pages (from-to)	2278-2282
Number of pages	5
Journal	FASEB Journal
Volume	2
Issue number	7
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Cell Biology

Cite this

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title = "Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells",

abstract = "A marked increase in cellular ATP consumption was induced by verapamil in the multidrug-resistant (MDR) cell line 2780(AD), but not in the drug-sensitive parental cell line A2780. A group of structurally unrelated drugs in concentrations known to reverse MDR, but not the verapamil analog tiapamil, a weak modulator of MDR, had similar effects. This effect was saturated at verapamil concentrations of about 1 μM. These data demonstrate that verapamil concentrations in MDR cells are maintained at a low level at the expense of ATP hydrolysis, and provide a first indication of the amount of metabolic energy used in this process.",

author = "Broxterman, {H. J.} and Pinedo, {H. M.} and Kuiper, {C. M.} and Kaptein, {L. C M} and Schuurhuis, {G. J.} and J. Lankelma",

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T1 - Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells

AU - Broxterman, H. J.

AU - Pinedo, H. M.

AU - Kuiper, C. M.

AU - Kaptein, L. C M

AU - Schuurhuis, G. J.

AU - Lankelma, J.

PY - 1988

Y1 - 1988

N2 - A marked increase in cellular ATP consumption was induced by verapamil in the multidrug-resistant (MDR) cell line 2780(AD), but not in the drug-sensitive parental cell line A2780. A group of structurally unrelated drugs in concentrations known to reverse MDR, but not the verapamil analog tiapamil, a weak modulator of MDR, had similar effects. This effect was saturated at verapamil concentrations of about 1 μM. These data demonstrate that verapamil concentrations in MDR cells are maintained at a low level at the expense of ATP hydrolysis, and provide a first indication of the amount of metabolic energy used in this process.

AB - A marked increase in cellular ATP consumption was induced by verapamil in the multidrug-resistant (MDR) cell line 2780(AD), but not in the drug-sensitive parental cell line A2780. A group of structurally unrelated drugs in concentrations known to reverse MDR, but not the verapamil analog tiapamil, a weak modulator of MDR, had similar effects. This effect was saturated at verapamil concentrations of about 1 μM. These data demonstrate that verapamil concentrations in MDR cells are maintained at a low level at the expense of ATP hydrolysis, and provide a first indication of the amount of metabolic energy used in this process.

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Induction by verapamil of a rapid increase in ATP consumption in multidrug-resistant tumor cells

Abstract

ASJC Scopus subject areas

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