Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes

Jean E. Merrill, Sean P. Murphy, Branislava Mitrovic, Allan Mackenzie-Graham, Joel C. Dopp, Minzhen Ding, Jeannette Griscavage, Louis J. Ignarro, Charles J. Lowenstein

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131-kDa iNOS protein by Western blot analysis; protein was also detected in cells by single- and double-label immunohistochemistry for iNOS and the oligodendrocyte-specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin-independent NO production in these in vitro cultures.

Original languageEnglish (US)
Pages (from-to)372-384
Number of pages13
JournalJournal of Neuroscience Research
Issue number4
StatePublished - May 15 1997


  • in situ hybridization
  • multiple sclerosis
  • nitric oxide

ASJC Scopus subject areas

  • Neuroscience(all)


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