TY - JOUR
T1 - Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes
AU - Merrill, Jean E.
AU - Murphy, Sean P.
AU - Mitrovic, Branislava
AU - Mackenzie-Graham, Allan
AU - Dopp, Joel C.
AU - Ding, Minzhen
AU - Griscavage, Jeannette
AU - Ignarro, Louis J.
AU - Lowenstein, Charles J.
PY - 1997/5/15
Y1 - 1997/5/15
N2 - It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131-kDa iNOS protein by Western blot analysis; protein was also detected in cells by single- and double-label immunohistochemistry for iNOS and the oligodendrocyte-specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin-independent NO production in these in vitro cultures.
AB - It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131-kDa iNOS protein by Western blot analysis; protein was also detected in cells by single- and double-label immunohistochemistry for iNOS and the oligodendrocyte-specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin-independent NO production in these in vitro cultures.
KW - in situ hybridization
KW - multiple sclerosis
KW - nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=0030996136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030996136&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4547(19970515)48:4<372::AID-JNR9>3.0.CO;2-8
DO - 10.1002/(SICI)1097-4547(19970515)48:4<372::AID-JNR9>3.0.CO;2-8
M3 - Article
C2 - 9169863
AN - SCOPUS:0030996136
SN - 0360-4012
VL - 48
SP - 372
EP - 384
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 4
ER -