TY - JOUR
T1 - Increases in Sequence Specific DNA Binding by p53 following Treatment with Chemotherapeutic and DNA Damaging Agents1
AU - Tishler, Roy B.
AU - Calderwood, Stuart K.
AU - Coleman, C. Norman
AU - Price, Brendan D.
PY - 1993/5
Y1 - 1993/5
N2 - We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.
AB - We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.
UR - http://www.scopus.com/inward/record.url?scp=0027212267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027212267&partnerID=8YFLogxK
M3 - Article
C2 - 8485705
AN - SCOPUS:0027212267
SN - 0008-5472
VL - 53
SP - 2212
EP - 2216
JO - Cancer Research
JF - Cancer Research
IS - 10
ER -