Increased interleukin-1 production and monocyte suppressor cell activity associated with human tuberculosis

H. Fujiwara, M. E. Kleinhenz, R. S. Wallis, J. J. Ellner

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Interleukin-1 (IL-1) production by blood monocytes and blastogenesis produced by tuberculin purified protein derivative (PPD) in blood T lymphocytes were examined in patients with pulmonary tuberculosis. Monocytes were isolated from blood mononuclear cells by plastic adherence, and IL-1 activity was determined in the mouse thymocyte proliferation assay. Monocytes from patients with tuberculosis produced significantly higher activities of IL-1 than did those from healthy tuberculin reactors when stimulated with lipopolysaccharide (LPS) (patients, 56.1 ± 20.0 U/ml; healthy control subjects, 7.3 ± 1.7 U/ml; p <0.05) or PPD (patients, 28.1 ± 7.2 U/ml; healthy control subjects, 9.5 ± 2.9 U/ml; p <0.05). In contrast, PPD-induced blastogenic responses in peripheral blood mononuclear cells (PBMC) from the patients were lower than those from healthy subjects (patients, 4,506 ± 1,145 cpm; healthy control subjects, 14,655 ± 2,240 cpmp; p <0.005), and IL-1 production by monocytes showed a positive correlation with monocyte suppressor activity for PPD-induced blastogenesis. Moreover, exogenous IL-1 was capable of suppressng antigen-induced blastogenesis of PBMC from healthy subjects. These data suggest that monocytes from patients with pulmonary tuberculosis are activated to produce or secrete increased levels of IL-1 and IL-1 may be a mediator of suppressor cell function.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalAmerican Review of Respiratory Disease
Issue number1
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine


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