Increased expression of hypoxia inducible factor-1α in rat and human prostate cancer

Hua Zhong, Faton Agani, Angelo A. Baccala, Erik Laughner, Natalia Rioseco-Camacho, William B. Isaacs, Jonathan W. Simons, Gregg L. Semenza

Research output: Contribution to journalArticlepeer-review

204 Scopus citations


Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that regulates genes involved in adaptation to hypoxia. Expression of HIF-1α was evaluated in rat and human prostate cancer cell lines. Increased expression of HIF-1α mRNA in rat prostate cancer cell lines and hypoxia-induced expression of HIF-1α, protein in human prostate cancer cell lines are associated with increased cell growth rates and metastatic potential. HIF- 1α mRNA was undetectable in the normal rat ventral prostate by Northern blot hybridization. HIF-1α protein expression and HIF-1 DNA binding activity were detected in normoxic PC-3 cells. Human prostate cancer cells plated at low density manifested higher functional HIF-1α expression than cells plated at high density independent of O2 tension. HIF-1α may become dysregulated in prostate cancer and thus drive the transcription of hypoxia-adaptive genes involved in tumor progression. This is also the first evidence that human cancer cells can express functional HIF-1α protein under normoxic conditions.

Original languageEnglish (US)
Pages (from-to)5280-5284
Number of pages5
JournalCancer Research
Issue number23
StatePublished - Dec 1 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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