Increased adherence of fluconazole-resistant isolates of Candida species to explanted esophageal mucosa

C. A. Lyman; K. F. Garrett; J. Peter; C. Gonzalez; T. J. Walsh

doi:10.1007/s100960050262

Increased adherence of fluconazole-resistant isolates of Candida species to explanted esophageal mucosa

C. A. Lyman, K. F. Garrett, J. Peter, C. Gonzalez, T. J. Walsh

School of Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The adherence of fluconazole-resistant and fluconazole-susceptible isolates of Candida albicans to explanted rabbit esophageal mucosa was examined in vivo. Among six Candida albicans isolates collected from HIV-infected patients, three fluconazole-resistant (MIC > 64 μg/ml) isolates attached more avidly than three fluconazole-susceptible strains (MIC ≤ 0.5 μg/ml) to esophageal mucosa (P ≤ 0.05). When three strains each of six different Candida spp. were compared, the more inherently fluconazole-resistant isolates adhered more avidly in the following order: Candida glabrata > Candida krusei > Candida albicans fluconazole-sensitive > Candida tropicalis > Candida parapsilosis. Nonetheless, fluconazole-resistant Candida albicans demonstrated the greatest degree of adherence in comparison to all fluconazole-susceptible Candida albicans (P < 0.001) and to all Candida spp. tested (P < 0.001). Thus, the refractoriness of esophageal candidiasis in patients infected with fluconazole-resistant isolates may be related to both in vitro drug resistance and increased mucosal adherence.

Original language	English (US)
Pages (from-to)	213-216
Number of pages	4
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	18
Issue number	3
DOIs	https://doi.org/10.1007/s100960050262
State	Published - 1999

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1007/s100960050262

Cite this

@article{809814f5891644328167bafcaf09aa36,

title = "Increased adherence of fluconazole-resistant isolates of Candida species to explanted esophageal mucosa",

abstract = "The adherence of fluconazole-resistant and fluconazole-susceptible isolates of Candida albicans to explanted rabbit esophageal mucosa was examined in vivo. Among six Candida albicans isolates collected from HIV-infected patients, three fluconazole-resistant (MIC > 64 μg/ml) isolates attached more avidly than three fluconazole-susceptible strains (MIC ≤ 0.5 μg/ml) to esophageal mucosa (P ≤ 0.05). When three strains each of six different Candida spp. were compared, the more inherently fluconazole-resistant isolates adhered more avidly in the following order: Candida glabrata > Candida krusei > Candida albicans fluconazole-sensitive > Candida tropicalis > Candida parapsilosis. Nonetheless, fluconazole-resistant Candida albicans demonstrated the greatest degree of adherence in comparison to all fluconazole-susceptible Candida albicans (P < 0.001) and to all Candida spp. tested (P < 0.001). Thus, the refractoriness of esophageal candidiasis in patients infected with fluconazole-resistant isolates may be related to both in vitro drug resistance and increased mucosal adherence.",

author = "Lyman, {C. A.} and Garrett, {K. F.} and J. Peter and C. Gonzalez and Walsh, {T. J.}",

note = "Funding Information: All organisms used were clinical isolates obtained from patients of the National Cancer Institute at the Warren Grant Magnuson Clinical Center, the National Institutes of Health. Identification of the isolates was made using standard microbiological techniques. Prior to use, aliquots from frozen stock cultures were streaked onto Sabouraud glucose agar and incubated at 37 °C for 24 h. Well-isolated colonies were subsequently inoculated into Emmon{\textquoteright}s modification of Sabouraud glucose broth and incubated in a gyrorotary water bath at 37 °C for 18 h, centrifuged, and washed with 0.154 M NaCl. The concentrations of blastoconidia were adjusted by hemacytometer counts. Minimum inhibitory concentrations of fluconazole for Candida isolates were determined using a standardized macrobroth dilution method [9].",

year = "1999",

doi = "10.1007/s100960050262",

language = "English (US)",

volume = "18",

pages = "213--216",

journal = "European Journal of Clinical Microbiology and Infectious Diseases",

issn = "0934-9723",

publisher = "Springer Verlag",

number = "3",

}

TY - JOUR

T1 - Increased adherence of fluconazole-resistant isolates of Candida species to explanted esophageal mucosa

AU - Lyman, C. A.

AU - Garrett, K. F.

AU - Peter, J.

AU - Gonzalez, C.

AU - Walsh, T. J.

N1 - Funding Information: All organisms used were clinical isolates obtained from patients of the National Cancer Institute at the Warren Grant Magnuson Clinical Center, the National Institutes of Health. Identification of the isolates was made using standard microbiological techniques. Prior to use, aliquots from frozen stock cultures were streaked onto Sabouraud glucose agar and incubated at 37 °C for 24 h. Well-isolated colonies were subsequently inoculated into Emmon’s modification of Sabouraud glucose broth and incubated in a gyrorotary water bath at 37 °C for 18 h, centrifuged, and washed with 0.154 M NaCl. The concentrations of blastoconidia were adjusted by hemacytometer counts. Minimum inhibitory concentrations of fluconazole for Candida isolates were determined using a standardized macrobroth dilution method [9].

PY - 1999

Y1 - 1999

N2 - The adherence of fluconazole-resistant and fluconazole-susceptible isolates of Candida albicans to explanted rabbit esophageal mucosa was examined in vivo. Among six Candida albicans isolates collected from HIV-infected patients, three fluconazole-resistant (MIC > 64 μg/ml) isolates attached more avidly than three fluconazole-susceptible strains (MIC ≤ 0.5 μg/ml) to esophageal mucosa (P ≤ 0.05). When three strains each of six different Candida spp. were compared, the more inherently fluconazole-resistant isolates adhered more avidly in the following order: Candida glabrata > Candida krusei > Candida albicans fluconazole-sensitive > Candida tropicalis > Candida parapsilosis. Nonetheless, fluconazole-resistant Candida albicans demonstrated the greatest degree of adherence in comparison to all fluconazole-susceptible Candida albicans (P < 0.001) and to all Candida spp. tested (P < 0.001). Thus, the refractoriness of esophageal candidiasis in patients infected with fluconazole-resistant isolates may be related to both in vitro drug resistance and increased mucosal adherence.

AB - The adherence of fluconazole-resistant and fluconazole-susceptible isolates of Candida albicans to explanted rabbit esophageal mucosa was examined in vivo. Among six Candida albicans isolates collected from HIV-infected patients, three fluconazole-resistant (MIC > 64 μg/ml) isolates attached more avidly than three fluconazole-susceptible strains (MIC ≤ 0.5 μg/ml) to esophageal mucosa (P ≤ 0.05). When three strains each of six different Candida spp. were compared, the more inherently fluconazole-resistant isolates adhered more avidly in the following order: Candida glabrata > Candida krusei > Candida albicans fluconazole-sensitive > Candida tropicalis > Candida parapsilosis. Nonetheless, fluconazole-resistant Candida albicans demonstrated the greatest degree of adherence in comparison to all fluconazole-susceptible Candida albicans (P < 0.001) and to all Candida spp. tested (P < 0.001). Thus, the refractoriness of esophageal candidiasis in patients infected with fluconazole-resistant isolates may be related to both in vitro drug resistance and increased mucosal adherence.

UR - http://www.scopus.com/inward/record.url?scp=0032754693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032754693&partnerID=8YFLogxK

U2 - 10.1007/s100960050262

DO - 10.1007/s100960050262

M3 - Article

C2 - 10357058

AN - SCOPUS:0032754693

SN - 0934-9723

VL - 18

SP - 213

EP - 216

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

IS - 3

ER -

Increased adherence of fluconazole-resistant isolates of Candida species to explanted esophageal mucosa

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this