Abstract
Purpose: We compared the use of near-infrared conjugates of 2-deoxyglucose (NIR 2-DG) to 2-deoxy-2-[18 F]fluoro-D-glucose (18 F-FDG) for the purposes of imaging tumors, as well as response to therapy. Procedures: Uptake of both 18 F-FDG and NIR 2-DG within gastrointestinal stromal tumor xenografts were imaged before and after nilotinib treatment. Confocal microscopy was performed to determine NIR 2-DG distribution in tumors. Results: Treatment with nilotinib resulted in a rapid reduction in 18 F-FDG uptake and reduced tumor cell viability which was predictive of long-term antitumor efficacy. In contrast, optical imaging with NIR 2-DG probes was unable to differentiate control from niltonib-treated animals, and microscopic analysis revealed no change in probe distribution as a result of treatment. Conclusions: These results suggest that conjugation of large bulky fluorophores to 2-DG disrupts the facilitated transport and retention of these probes in cells. Therefore, optical imaging of NIR 2-DG probes cannot substitute for 18 F-FDG positron emission tomography imaging as a biomarker of tumor cell viability and metabolism.
Original language | English (US) |
---|---|
Pages (from-to) | 553-560 |
Number of pages | 8 |
Journal | Molecular Imaging and Biology |
Volume | 14 |
Issue number | 5 |
DOIs | |
State | Published - Oct 2012 |
Keywords
- 2-Deoxyglucose (2-DG)
- F-FDG
- Fluorescent imaging
- Gastrointestinal tumor (GIST)
- MicroPET
- Near-infrared
- Nilotinib
- Optical imaging
- Tumor xenograft
ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Cancer Research