In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration

Saijilafu; Bo Yin Zhang; Feng Quan Zhou

doi:10.1007/978-1-4939-0777-9_14

In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration

Saijilafu, Bo Yin Zhang, Feng Quan Zhou

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

Original language	English (US)
Pages (from-to)	167-175
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1162
DOIs	https://doi.org/10.1007/978-1-4939-0777-9_14
State	Published - 2014

Keywords

Adult mouse sensory neurons
Axon regeneration
Dorsal root ganglion
In vivo electroporation
Peripheral nervous system

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0777-9_14

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title = "In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration",

abstract = "Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.",

keywords = "Adult mouse sensory neurons, Axon regeneration, Dorsal root ganglion, In vivo electroporation, Peripheral nervous system",

author = "Saijilafu and Zhang, {Bo Yin} and Zhou, {Feng Quan}",

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AU - Saijilafu,

AU - Zhang, Bo Yin

AU - Zhou, Feng Quan

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PY - 2014

Y1 - 2014

N2 - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

AB - Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain-and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

KW - Adult mouse sensory neurons

KW - Axon regeneration

KW - Dorsal root ganglion

KW - In vivo electroporation

KW - Peripheral nervous system

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ER -

In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration

Abstract

Keywords

ASJC Scopus subject areas

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