TY - JOUR
T1 - In vivo assessment of the placental anatomy and perfusion in a mouse model of intrauterine inflammation
AU - Wu, Dan
AU - Lei, Jun
AU - Jia, Bei
AU - Xie, Han
AU - Zhu, Yan
AU - Xu, Jiadi
AU - Mori, Susumu
AU - Zhang, Jiangyang
AU - Burd, Irina
N1 - Funding Information:
Contract grant sponsor: National Institute of Neurological Disorders and Stroke (NINDS), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD); contract grant number: R21 NS098018, K08 HD073315, and R01 HD074593
Publisher Copyright:
© 2017 International Society for Magnetic Resonance in Medicine
PY - 2018/5
Y1 - 2018/5
N2 - Background: Magnetic resonance imaging (MRI) provides useful markers to examine placental function. MRI features of placental injury due to intrauterine inflammation—a common risk during pregnancy, are not well known. Purpose: To investigate the capability of structural MRI and intravoxel incoherent motion (IVIM) imaging in examining acute placental injury in a mouse model of intrauterine inflammation, as well as gestation-dependent placental changes. Study Type: Prospective study. Animal Model: Pregnant CD1 mice were scanned on embryonic day 15 (E15, n = 40 placentas from six dams) and E17. On E17, mice were subjected to intrauterine injury by exposure to lipopolysaccharide (LPS, n = 25 placentas from three dams) or sham injury (n = 25 placentas from three dams). Field Strength/Sequence: In vivo MRI was performed on an 11.7T Bruker scanner, using a fast spin-echo sequence and a diffusion-weighted echo-planar imaging (EPI) sequence. Assessment: T 2 -weighted MRI was acquired to evaluate placental volume. IVIM imaging was performed in a restricted field-of-view using 15 b-values from 10–800 s/mm 2 , based on which, the pseudodiffusion fraction (f), pseudodiffusion coefficient (D*), and tissue water coefficient (D) were estimated with a two-step fitting procedure. Statistical Tests: Two-way analysis of variance (ANOVA) was used to evaluate the group differences. Results: The placental volume increased by ∼21% from E15 to E17 (P < 0.01), and a 15% volume loss was observed at 6 hours after LPS exposure (P < 0.01). IVIM parameters (f, D*, and f·D*) were similar between the E15 and E17 sham groups (P > 0.05), which was significantly reduced in the LPS-exposed placentas compared to the shams (P < 0.001). D values decreased from E15 to E17 (P < 0.05), which were further reduced after LPS exposure (P < 0.05). Changes in placental area and vascular density were histologically identified in the LPS-exposed group, along with gestation-dependent changes. Data Conclusion: Our results suggested structural MRI and IVIM measurements are potential markers for detecting acute placental injury after intrauterine inflammation. Level of Evidence: 1. Technical Efficacy: Stage 2. J. Magn. Reson. Imaging 2018;47:1260–1267.
AB - Background: Magnetic resonance imaging (MRI) provides useful markers to examine placental function. MRI features of placental injury due to intrauterine inflammation—a common risk during pregnancy, are not well known. Purpose: To investigate the capability of structural MRI and intravoxel incoherent motion (IVIM) imaging in examining acute placental injury in a mouse model of intrauterine inflammation, as well as gestation-dependent placental changes. Study Type: Prospective study. Animal Model: Pregnant CD1 mice were scanned on embryonic day 15 (E15, n = 40 placentas from six dams) and E17. On E17, mice were subjected to intrauterine injury by exposure to lipopolysaccharide (LPS, n = 25 placentas from three dams) or sham injury (n = 25 placentas from three dams). Field Strength/Sequence: In vivo MRI was performed on an 11.7T Bruker scanner, using a fast spin-echo sequence and a diffusion-weighted echo-planar imaging (EPI) sequence. Assessment: T 2 -weighted MRI was acquired to evaluate placental volume. IVIM imaging was performed in a restricted field-of-view using 15 b-values from 10–800 s/mm 2 , based on which, the pseudodiffusion fraction (f), pseudodiffusion coefficient (D*), and tissue water coefficient (D) were estimated with a two-step fitting procedure. Statistical Tests: Two-way analysis of variance (ANOVA) was used to evaluate the group differences. Results: The placental volume increased by ∼21% from E15 to E17 (P < 0.01), and a 15% volume loss was observed at 6 hours after LPS exposure (P < 0.01). IVIM parameters (f, D*, and f·D*) were similar between the E15 and E17 sham groups (P > 0.05), which was significantly reduced in the LPS-exposed placentas compared to the shams (P < 0.001). D values decreased from E15 to E17 (P < 0.05), which were further reduced after LPS exposure (P < 0.05). Changes in placental area and vascular density were histologically identified in the LPS-exposed group, along with gestation-dependent changes. Data Conclusion: Our results suggested structural MRI and IVIM measurements are potential markers for detecting acute placental injury after intrauterine inflammation. Level of Evidence: 1. Technical Efficacy: Stage 2. J. Magn. Reson. Imaging 2018;47:1260–1267.
KW - gestation
KW - intrauterine inflammation
KW - intravoxel-incoherent motion
KW - placental perfusion
KW - placental volume
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U2 - 10.1002/jmri.25867
DO - 10.1002/jmri.25867
M3 - Article
C2 - 28981189
AN - SCOPUS:85030543813
SN - 1053-1807
VL - 47
SP - 1260
EP - 1267
JO - Journal of Magnetic Resonance Imaging
JF - Journal of Magnetic Resonance Imaging
IS - 5
ER -