@inbook{a47f6202ac73412180150bd1c33598b7,
title = "In vitro transcription from plasmid or PCR-amplified DNA",
abstract = "This protocol describes the synthesis and purification of RNAs using plasmid DNA or PCR-amplified DNA as a template. This procedure should give NTP-free, full-length RNA for all sizes of RNA. This protocol is derived from Milligan and Uhlenbeck, the classic paper on T7 transcription reactions, with modifications.",
keywords = "Column purification, DNA, DNA Phenol extract, In vitro transcription reaction, PCR-amplified, RNA polymerase, Template",
author = "Brunelle, {Julie L.} and Rachel Green",
year = "2013",
doi = "10.1016/B978-0-12-420037-1.00005-1",
language = "English (US)",
isbn = "9780124200371",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "101--114",
booktitle = "Laboratory Methods in Enzymology",
}