In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents

Darrell Abernethy; Charles Hignite; Daniel L. Azarnoff

doi:10.1016/0039-128X(76)90052-0

In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents

Darrell Abernethy, Charles Hignite, Daniel L. Azarnoff

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In vitro conversion of 2-¹⁴C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.

Original language	English (US)
Pages (from-to)	297-307
Number of pages	11
Journal	Steroids
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/0039-128X(76)90052-0
State	Published - Mar 1976

Keywords

24-Dihydrolanosterol
5α-lanost-8en-33-ol
5α-lanosta-8,24-dien-3β-ol
Cholest-5en-3β-ol
Cholesterol
Lanosterol

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/0039-128X(76)90052-0

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@article{c5f9b18fb7d244659c9d96d3d4384807,

title = "In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents",

abstract = "In vitro conversion of 2-14C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.",

keywords = "24-Dihydrolanosterol, 5α-lanost-8en-33-ol, 5α-lanosta-8,24-dien-3β-ol, Cholest-5en-3β-ol, Cholesterol, Lanosterol",

author = "Darrell Abernethy and Charles Hignite and Azarnoff, {Daniel L.}",

note = "Funding Information: This work was supported by the United States Public Health Service Grant, GM 15956, and the Pharmaceutical Manufacturers Association Foundation.",

year = "1976",

month = mar,

doi = "10.1016/0039-128X(76)90052-0",

language = "English (US)",

volume = "27",

pages = "297--307",

journal = "Steroids",

issn = "0039-128X",

publisher = "Elsevier Inc.",

number = "3",

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T1 - In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents

AU - Abernethy, Darrell

AU - Hignite, Charles

AU - Azarnoff, Daniel L.

N1 - Funding Information: This work was supported by the United States Public Health Service Grant, GM 15956, and the Pharmaceutical Manufacturers Association Foundation.

PY - 1976/3

Y1 - 1976/3

N2 - In vitro conversion of 2-14C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.

AB - In vitro conversion of 2-14C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.

KW - 24-Dihydrolanosterol

KW - 5α-lanost-8en-33-ol

KW - 5α-lanosta-8,24-dien-3β-ol

KW - Cholest-5en-3β-ol

KW - Cholesterol

KW - Lanosterol

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U2 - 10.1016/0039-128X(76)90052-0

DO - 10.1016/0039-128X(76)90052-0

M3 - Article

C2 - 1265795

AN - SCOPUS:0017293809

SN - 0039-128X

VL - 27

SP - 297

EP - 307

JO - Steroids

JF - Steroids

IS - 3

ER -

In vitro inhibition of rat liver cholest-5en-3β-ol (cholesterol) biosynthesis by non-mercurial sulfhydryl reagents

Abstract

Keywords

ASJC Scopus subject areas

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