Abstract
In vitro conversion of 2-14C-mevalonate to cholest-5en-3β-ol (cholesterol) in rat liver homogenates is inhibited by arsenite, β-mercaptoethanol, dithiothreitol and ethanethiol. Two sterols containing 20 carbon atoms accumulate under these conditions. One of these is identified as 4,4 dimethyl-5α-cholest-8en-3β-ol and the other tentatively identified as 4,4 dimethyl-5α-cholest-8,24-dien-3β-ol. Based on these observations, these non-mercurial sulfhydryl reagents do not inhibit 5α-lanosta-8,24-dien-3β-ol 14α demethylase.
Original language | English (US) |
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Pages (from-to) | 297-307 |
Number of pages | 11 |
Journal | Steroids |
Volume | 27 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1976 |
Keywords
- 24-Dihydrolanosterol
- 5α-lanost-8en-33-ol
- 5α-lanosta-8,24-dien-3β-ol
- Cholest-5en-3β-ol
- Cholesterol
- Lanosterol
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology
- Pharmacology
- Clinical Biochemistry
- Organic Chemistry