In Vitro Growth of Pure Cultures of Retinal Pigment Epithelium

Daniel M. Albert, MAJ Mark O.M. Tso, Alan S. Rabson

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Retinal pigment epithelium (RPE) was isolated from five human eyes and grown in vitro as an explant culture. Cultures of choroid and RPE with choroid were similarly established for comparison. The tissues were maintained in culture for periods up to six months, and the morphologic characteristics of the cells were studied and documented. Mosaic, tubular, and spindle growth patterns were observed to evolve from the RPE. Certain cytologic features suggestive of dedifferentiation and neoplasia were noted. There was a general decrease in the amount of pigment in the cells as tissue culture progressed. The appearance of the cells grown in tissue culture is compared with that of RPE seen in normal and pathologic situations.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalArchives of ophthalmology
Issue number1
StatePublished - Jul 1972
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


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