Abstract
Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.
Original language | English (US) |
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Pages (from-to) | 293-306 |
Number of pages | 14 |
Journal | Cellular Immunology |
Volume | 54 |
Issue number | 2 |
DOIs | |
State | Published - Jan 1 1980 |
ASJC Scopus subject areas
- Immunology