In vitro growth of murine T cells: IV. Use of T-cell growth factor to clone lymphoid cells

Steven A. Rosenberg; Paul J. Spiess; Susan Schwarz

doi:10.1016/0008-8749(80)90211-7

In vitro growth of murine T cells: IV. Use of T-cell growth factor to clone lymphoid cells

Steven A. Rosenberg, Paul J. Spiess, Susan Schwarz

School of Medicine

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21 Scopus citations

Abstract

Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.

Original language	English (US)
Pages (from-to)	293-306
Number of pages	14
Journal	Cellular Immunology
Volume	54
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(80)90211-7
State	Published - Jan 1 1980

ASJC Scopus subject areas

Immunology

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abstract = "Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.",

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AB - Soft agar and limiting dilution techniques have been used in conjunction with T-cell growth factor to develop methods for the cloning of continuous T-lymphoid populations from single lymphoid cells. Clones have been produced from both fresh murine splenocytes as well as from in vitro sensitized cells. These T-cell clones have continued to express specific cytotoxic functions for over 4 months and should be a valuable source of monoclonal lymphoid cells for immunologic studies.

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In vitro growth of murine T cells: IV. Use of T-cell growth factor to clone lymphoid cells

Abstract

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