Abstract
We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA molecules consisting of an ATP-binding domain flanked by regions of random sequence. Different classes of kinases catalyse the transfer of the γ-thiophosphate of ATP-γS to the 5′-hydroxyl or to internal 2′-hydroxyls. An engineered version of one class is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.
Original language | English (US) |
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Pages (from-to) | 31-36 |
Number of pages | 6 |
Journal | Nature |
Volume | 371 |
Issue number | 6492 |
DOIs | |
State | Published - Jan 1 1994 |
ASJC Scopus subject areas
- General