@inproceedings{cbc8076115f14ff2b0d955b8204facd0,
title = "Improving the sensitivity of bacteria detection and quantification in urine samples via sample dilution and filtration",
abstract = "The development of rapid diagnostic methods is essential to ensure targeted treatments for common bacterial infections including urinary tract infections (UTI). To address this, we present a microfluidic droplet platform for rapid identification and quantification of bacterial uropathogens in human urine. We employ a nucleic acid amplification-free assay that relies on peptide nucleic acid (PNA) probes to specifically detect 16S ribosomal RNA from single bacteria in droplets. Furthermore, we improve the compatibility of our platform with a wide array of urine samples by employing dilution and filtration strategies to combat the effects of auto-fluorescence and particulates.",
keywords = "Bacteria, Dilution, Droplet, Filtration, Urine",
author = "Kaushik, \{Aniruddha M.\} and Kuangwen Hsieh and Wang, \{Tza Huei\}",
note = "Funding Information: We thank Dr. Karen Carroll and Michael Gherna at the JHU Clinical Microbiology Lab for providing us with clinical urine samples. We also thank our funding agencies, NIH (R01AI117032) and NSF (1033744). Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",
year = "2018",
language = "English (US)",
series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
publisher = "Chemical and Biological Microsystems Society",
pages = "1758--1761",
booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
}