Improvements in the RT-PCR detection of enteric viruses in environmental samples

K. J. Schwab; F. H. Neill; M. K. Estes; R. L. Atmar

doi:10.1016/S0273-1223(98)00805-1

Improvements in the RT-PCR detection of enteric viruses in environmental samples

K. J. Schwab, F. H. Neill, M. K. Estes, R. L. Atmar

Research output: Contribution to journal › Conference article › peer-review

13 Scopus citations

Abstract

The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.

Original language	English (US)
Pages (from-to)	83-86
Number of pages	4
Journal	Water Science and Technology
Volume	38
Issue number	12
DOIs	https://doi.org/10.1016/S0273-1223(98)00805-1
State	Published - 1998
Externally published	Yes
Event	Proceedings of the 1998 19th Biennial Conference of the International Association on Water Quality,. Part 10 (of 10) - Vancouver, Can Duration: Jun 21 1998 → Jun 26 1998

ASJC Scopus subject areas

Environmental Engineering
Water Science and Technology

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10.1016/S0273-1223(98)00805-1

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title = "Improvements in the RT-PCR detection of enteric viruses in environmental samples",

abstract = "The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.",

author = "Schwab, {K. J.} and Neill, {F. H.} and Estes, {M. K.} and Atmar, {R. L.}",

year = "1998",

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T1 - Improvements in the RT-PCR detection of enteric viruses in environmental samples

AU - Schwab, K. J.

AU - Neill, F. H.

AU - Estes, M. K.

AU - Atmar, R. L.

PY - 1998

Y1 - 1998

N2 - The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.

AB - The current methods for the detection of nucleic acid from enteric viruses in environmental samples usually involve extensive concentration and purification of target viruses followed by RT-PCR amplification using two enzymes, reverse transcriptase and Taq polymerase. A modified method was developed to improve RT-PCR assays. The improved method was as sensitive in NV and HAV detection the traditional two-enzyme method. These improvements to viral nucleic acid detection have the potential to improve sensitivity, specificity and confidence in RT-PCR results.

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DO - 10.1016/S0273-1223(98)00805-1

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EP - 86

JO - Water Science and Technology

JF - Water Science and Technology

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T2 - Proceedings of the 1998 19th Biennial Conference of the International Association on Water Quality,. Part 10 (of 10)

Y2 - 21 June 1998 through 26 June 1998

ER -

Improvements in the RT-PCR detection of enteric viruses in environmental samples

Abstract

ASJC Scopus subject areas

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