Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography

R. W. Walker; L. R. Mandel; J. E. Kleinman; J. C. Gillin; R. J. Wyatt; W. J.A. Vandenheuvel

doi:10.1016/S0378-4347(00)81833-2

Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography

R. W. Walker, L. R. Mandel, J. E. Kleinman, J. C. Gillin, R. J. Wyatt, W. J.A. Vandenheuvel

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A gas-liquid chromatographic-mass spectrometric procedure is described for the assay of dimethyltryptamine (DMT) in whole blood. The use of a glass capillary column in combination with selective ion monitoring results in an assay with a high degree of specificity and sensitivity. 5-Methoxy-DMT is used as an internal standard and carrier in the isolation procedure. The superior resolving characteristics of the capillary column (as compared to previously employed packed columns) allows monitoring of the intense m/e 58 ion arising from the DMT side-chain. A sensitivity limit of 10 pg/ml blood is realized with a 10-ml blood sample.

Original language	English (US)
Pages (from-to)	539-546
Number of pages	8
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	162
Issue number	4
DOIs	https://doi.org/10.1016/S0378-4347(00)81833-2
State	Published - Apr 11 1979
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1016/S0378-4347(00)81833-2

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Walker, R. W., Mandel, L. R., Kleinman, J. E., Gillin, J. C., Wyatt, R. J., & Vandenheuvel, W. J. A. (1979). Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 162(4), 539-546. https://doi.org/10.1016/S0378-4347(00)81833-2

Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography. / Walker, R. W.; Mandel, L. R.; Kleinman, J. E. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 162, No. 4, 11.04.1979, p. 539-546.

Research output: Contribution to journal › Article › peer-review

Walker, RW, Mandel, LR, Kleinman, JE, Gillin, JC, Wyatt, RJ & Vandenheuvel, WJA 1979, 'Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 162, no. 4, pp. 539-546. https://doi.org/10.1016/S0378-4347(00)81833-2

Walker RW, Mandel LR, Kleinman JE, Gillin JC, Wyatt RJ, Vandenheuvel WJA. Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography. Journal of Chromatography B: Biomedical Sciences and Applications. 1979 Apr 11;162(4):539-546. doi: 10.1016/S0378-4347(00)81833-2

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abstract = "A gas-liquid chromatographic-mass spectrometric procedure is described for the assay of dimethyltryptamine (DMT) in whole blood. The use of a glass capillary column in combination with selective ion monitoring results in an assay with a high degree of specificity and sensitivity. 5-Methoxy-DMT is used as an internal standard and carrier in the isolation procedure. The superior resolving characteristics of the capillary column (as compared to previously employed packed columns) allows monitoring of the intense m/e 58 ion arising from the DMT side-chain. A sensitivity limit of 10 pg/ml blood is realized with a 10-ml blood sample.",

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AU - Kleinman, J. E.

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AU - Wyatt, R. J.

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AB - A gas-liquid chromatographic-mass spectrometric procedure is described for the assay of dimethyltryptamine (DMT) in whole blood. The use of a glass capillary column in combination with selective ion monitoring results in an assay with a high degree of specificity and sensitivity. 5-Methoxy-DMT is used as an internal standard and carrier in the isolation procedure. The superior resolving characteristics of the capillary column (as compared to previously employed packed columns) allows monitoring of the intense m/e 58 ion arising from the DMT side-chain. A sensitivity limit of 10 pg/ml blood is realized with a 10-ml blood sample.

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Improved selective ion monitoring mass-spectrometric assay for the determination of n,n-dimethyltryptamine in human blood utilizing capillary column gas chromatography

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