Improved quantification from 1H-NMR spectra using reduced repetition times

Santosh Kumar Bharti, Neeraj Sinha, Bhawani Shankar Joshi, Sudhir Kumar Mandal, Raja Roy, Chunni Lal Khetrapal

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A method of choosing the correction factor based on Bloch equation for the quantitative estimation of metabolite from 1H NMR spectra recorded with reduced recycle delay is prescribed. The procedure reduces the experimental time without substantially compromising the accuracy of quantitative estimation. It is based on choosing the correction factors, which depend on T1 and T2 of the metabolite and recycle delay used for recording the spectra. It is validated by studying a mixture of amino acids with known concentration of constituents and human serum sample and it provides accuracy of quantitative estimation to 95-96%.

Original languageEnglish (US)
Pages (from-to)367-376
Number of pages10
Issue number4
StatePublished - 2008
Externally publishedYes


  • Body fluids
  • CPMG
  • Metabolites
  • NMR spectroscopy
  • Quantitation
  • T and T relaxation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry


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