Improved molecular technique for the differentiation of neotropical anopheline species

Ryan Matson, Carlos Tong Rios, Cesar Banda Chavez, Robert H. Gilman, David Florin, Victor Lopez Sifuentes, Roldan Cardenas Greffa, Pablo Peñataro Yori, Roberto Fernandez, Daniel Velasquez Portocarrero, Joseph M. Vinetz, Margaret Kosek

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We evaluated a PCR-RFLP of the ribosomal internal transcribed spacer 2 region (ITS2) to distinguish species of Anopheles commonly reported in the Amazon and validated this method using reared F1 offspring. The following species of Anopheles were used for molecular analysis: An. (Nys.) benarrochi, An. (Nys.) darlingi, An. (Nys.) nuneztovari, An. (Nys.) konderi, An. (Nys.) rangeli, and An. (Nys.) triannulatus sensu lato (s.l.). In addition, three species of the subgenus Anopheles, An. (Ano.) forattini, An. (Ano.) mattogrossensis, and An. (Ano.) peryassui were included for testing. Each of the nine species tested yielded diagnostic banding patterns. The PCR-RFLP method was successful in identifying all life stages including exuviae with small fractions of the sample. The assay is rapid and can be applied as an unbiased confirmatory method for identification of morphologic variants, disputed samples, imperfectly preserved specimens, and life stages from which taxonomic keys do not allow for definitive species determination.

Original languageEnglish (US)
Pages (from-to)492-498
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number3
StatePublished - Mar 2008

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases


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