TY - JOUR
T1 - Improved estimation of the distribution of suppressed plasma HIV-1 RNA in men receiving effective antiretroviral therapy
AU - Schneider, Michael F.
AU - Margolick, Joseph B.
AU - Jacobson, Lisa P.
AU - Reddy, Susheel
AU - Martinez-Maza, Otoniel
AU - Muñoz, Alvaro
PY - 2012/4/1
Y1 - 2012/4/1
N2 - Plasma HIV-1 RNA was measured in 306 samples, collected from 273 highly active antiretroviral therapy-experienced men, using both the Roche COBAS TaqMan (limit of detection = 20 copies per milliliter) and Roche Amplicor (limit of detection = 50 copies per milliliter) assays. Mixtures of Gaussian distributions incorporating left-censored data were used in analyses. The more sensitive TaqMan assay estimated that 23% and 0.0003% of HIV-1 RNA values would be below 1 copy per milliliter and 1 copy per 3 L, respectively. This is in sharp contrast to the overestimation provided by the less sensitive Amplicor assay, whereby the corresponding predicted percentages were 51% and 1%. Both assays appropriately characterized suboptimal virologic response as the rightmost peaks of both distributions provided an excellent fit to the observed data. Our results based on a widely available 20 copies per milliliter sensitive assay reproduce those obtained using customized assays that quantified HIV-1 RNA values as low as 1 copy per milliliter.
AB - Plasma HIV-1 RNA was measured in 306 samples, collected from 273 highly active antiretroviral therapy-experienced men, using both the Roche COBAS TaqMan (limit of detection = 20 copies per milliliter) and Roche Amplicor (limit of detection = 50 copies per milliliter) assays. Mixtures of Gaussian distributions incorporating left-censored data were used in analyses. The more sensitive TaqMan assay estimated that 23% and 0.0003% of HIV-1 RNA values would be below 1 copy per milliliter and 1 copy per 3 L, respectively. This is in sharp contrast to the overestimation provided by the less sensitive Amplicor assay, whereby the corresponding predicted percentages were 51% and 1%. Both assays appropriately characterized suboptimal virologic response as the rightmost peaks of both distributions provided an excellent fit to the observed data. Our results based on a widely available 20 copies per milliliter sensitive assay reproduce those obtained using customized assays that quantified HIV-1 RNA values as low as 1 copy per milliliter.
KW - Bimodal distribution
KW - HAART
KW - HIV-1 RNA
KW - Limit of detection
UR - http://www.scopus.com/inward/record.url?scp=84859740217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859740217&partnerID=8YFLogxK
U2 - 10.1097/QAI.0b013e318246bfce
DO - 10.1097/QAI.0b013e318246bfce
M3 - Article
C2 - 22217679
AN - SCOPUS:84859740217
SN - 1525-4135
VL - 59
SP - 389
EP - 392
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
IS - 4
ER -