Impact of tuberculosis (TB) on HIV-1 activity in dually infected patients

Z. Toossi, H. Mayanja-Kizza, C. S. Hirsch, K. L. Edmonds, T. Spahlinger, D. L. Hom, H. Aung, P. Mugyenyi, J. J. Ellner, C. W. Whalen

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126 Scopus citations


Active TB in HIV-I-infected subjects is associated with increased HIV-1-related immunodeficiency and mortality. We assessed plasma viral load in HIV-1-infected patients with pulmonary TB (HIV/TB) and non-TB symptomatic HIV-1-infected patients (HIV). HIV-1 load was higher in HIV/TB compared with HIV at higher CD4 counts (> 500/μ1) (P <0.01), but not at lower CD4 counts (<500/μ1). We also evaluated the status of HIV-1 gene expression in peripheral blood mononuclear cells (PBMC) and serum from HIV/TB and CD4-matched healthy HIV-infected patients (HIV/C) by reverse transcriptase-polymerase chain reaction over a range of CD4 (> 900/μ1 to <200/μ1). HIV-1 RNA in serum and PBMC correlated to one another, and both were markedly higher in HIV/TB compared with HIV/C with higher CD4 counts. Also, during a longitudinal study of anti-tuberculous chemoprophylaxis in HIV-1-infected patients, 10 subjects who developed TB had serologies before, at the time, and after the diagnosis of TB. These HIV/TB patients had an increase in viral load (average 2.5-fold) at the time of diagnosis of TB (P <0.05). Overall, these data indicate that the transcriptional activity of HIV-1 is enhanced in HIV-1-infected patients with active TB, especially during early HIV-1 disease. As TB often is an early HIV-1 opportunistic infection, it may particularly favour early viral replication and dissemination, and therefore contribute to progression of HIV-1 disease.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalClinical and Experimental Immunology
Issue number2
StatePublished - 2001
Externally publishedYes


  • HIV-1
  • Mycobacterium tuberculosis
  • Opportunistic infection
  • Transcriptional activation
  • Tumour necrosis factor-alpha

ASJC Scopus subject areas

  • Immunology


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