TY - JOUR
T1 - Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen
AU - Rittenour, William R.
AU - Hamilton, Robert G.
AU - Beezhold, Donald H.
AU - Green, Brett J.
N1 - Funding Information:
The findings and the conclusions in this report are those of the authors and do not necessarily represent the views of the National Institute for Occupational Safety and Health. This work was supported in part by an InterAgency agreement with the National Institute of Environmental Health Sciences ( Y1-ES0001-06 ).
PY - 2012/9/28
Y1 - 2012/9/28
N2 - Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen.
AB - Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen.
KW - Allergen
KW - Immunoassay
KW - Pollen
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=84864389754&partnerID=8YFLogxK
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U2 - 10.1016/j.jim.2012.01.012
DO - 10.1016/j.jim.2012.01.012
M3 - Review article
C2 - 22342607
AN - SCOPUS:84864389754
SN - 0022-1759
VL - 383
SP - 47
EP - 53
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -