TY - JOUR
T1 - Immunochemical studies of dextran coupled ragweed pollen allergen, antigen E
AU - Piao King, Te
AU - Kochoumian, Lucia
AU - Ishizaka, Kimishige
AU - Lichtenstein, Lawrence M.
AU - Norman, Philip S.
N1 - Funding Information:
‘This work was supported in part by Grants AI-08445, AI-07290 and AI-0866 of the National Institutes of Health, and by Grant GB-41443 from the National Science Foundation.
PY - 1975/8
Y1 - 1975/8
N2 - The major allergen of ragweed pollen antigen E has been coupled to periodate oxidized dextrans, followed with sodium borohydride reduction to stabilize the linkages. Two products having molecular weights of about 100,000 and 140,000 were prepared, and the molar ratio of dextran to antigen E in both products was the same in the range of 2-5. The antigenic and the allergenic activities of the products were on a molar basis about seven to eight fold less than those of antigen E. On dextranase digestion of the products, their biological activities were restored. The products were immunogenic in rabbits to stimulate the formation of antibodies reacting with antigen E and with dextran.
AB - The major allergen of ragweed pollen antigen E has been coupled to periodate oxidized dextrans, followed with sodium borohydride reduction to stabilize the linkages. Two products having molecular weights of about 100,000 and 140,000 were prepared, and the molar ratio of dextran to antigen E in both products was the same in the range of 2-5. The antigenic and the allergenic activities of the products were on a molar basis about seven to eight fold less than those of antigen E. On dextranase digestion of the products, their biological activities were restored. The products were immunogenic in rabbits to stimulate the formation of antibodies reacting with antigen E and with dextran.
UR - http://www.scopus.com/inward/record.url?scp=0016772587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016772587&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(75)90189-7
DO - 10.1016/0003-9861(75)90189-7
M3 - Article
C2 - 1237272
AN - SCOPUS:0016772587
SN - 0003-9861
VL - 169
SP - 464
EP - 473
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -