Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice

Julian Sanchez-Bautista; Catherine A. Foss; Alvaro A. Ordonez; Mariah H. Klunk; Sanjay K. Jain

doi:10.1007/s11307-018-1249-0

Imaging Pulmonary Foreign Body Reaction Using [¹²⁵I]iodo-DPA-713 SPECT/CT in Mice

Julian Sanchez-Bautista, Catherine A. Foss, Alvaro A. Ordonez, Mariah H. Klunk, Sanjay K. Jain

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [¹²⁵I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model. Procedures: C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [¹²⁵I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively. Results: Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [¹²⁵I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings. Conclusion: Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.

Original language	English (US)
Pages (from-to)	228-231
Number of pages	4
Journal	Molecular Imaging and Biology
Volume	21
Issue number	2
DOIs	https://doi.org/10.1007/s11307-018-1249-0
State	Published - Apr 15 2019

Keywords

Foreign body
Granulomatous inflammation
Iodo-DPA-713

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

Access to Document

10.1007/s11307-018-1249-0

Cite this

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title = "Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice",

abstract = "Purpose: Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model. Procedures: C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively. Results: Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings. Conclusion: Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.",

keywords = "Foreign body, Granulomatous inflammation, Iodo-DPA-713",

author = "Julian Sanchez-Bautista and Foss, {Catherine A.} and Ordonez, {Alvaro A.} and Klunk, {Mariah H.} and Jain, {Sanjay K.}",

note = "Publisher Copyright: {\textcopyright} 2018, World Molecular Imaging Society.",

year = "2019",

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day = "15",

doi = "10.1007/s11307-018-1249-0",

language = "English (US)",

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AU - Sanchez-Bautista, Julian

AU - Foss, Catherine A.

AU - Ordonez, Alvaro A.

AU - Klunk, Mariah H.

AU - Jain, Sanjay K.

PY - 2019/4/15

Y1 - 2019/4/15

N2 - Purpose: Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model. Procedures: C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively. Results: Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings. Conclusion: Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.

AB - Purpose: Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model. Procedures: C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively. Results: Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings. Conclusion: Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.

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