Imaging Enterobacterales infections in patients using pathogen-specific positron emission tomography

Alvaro A. Ordonez, Luz M. Wintaco, Filipa Mota, Andres F. Restrepo, Camilo A.Ruiz Bedoya, Carlos F. Reyes, Luis G. Uribe, Sudhanshu Abhishek, Franco R.D. Alessio, Daniel P. Holt, Robert F. Dannals, Steven P. Rowe, Victor R. Castillo, Martin G. Pomper, Ulises Granados, Sanjay K. Jain

Research output: Contribution to journalArticlepeer-review


Enterobacterales represent the largest group of bacterial pathogens in humans and are responsible for severe, deep-seated infections, often resulting in sepsis or death. They are also a prominent cause of multidrug-resistant (MDR) infections, and some species are recognized as biothreat pathogens. Tools for noninvasive, whole-body analysis that can localize a pathogen with specificity are needed, but no such technology currently exists. We previously demonstrated that positron emission tomography (PET) with 2-deoxy-2-[18F]fluoro-d-sorbitol (18F-FDS) can selectively detect Enterobacterales infections in murine models. Here, we demonstrate that uptake of 18F-FDS by bacteria occurs via a metabolically conserved sorbitol-specific pathway with rapid in vitro 18F-FDS uptake noted in clinical strains, including MDR isolates. Whole-body 18F-FDS PET/computerized tomography (CT) in 26 prospectively enrolled patients with either microbiologically confirmed Enterobacterales infection or other pathologies demonstrated that 18F-FDS PET/CT was safe, could rapidly detect and localize Enterobacterales infections due to drug-susceptible or MDR strains, and differentiated them from sterile inflammation or cancerous lesions. Repeat imaging in the same patients monitored antibiotic efficacy with decreases in PET signal correlating with clinical improvement. To facilitate the use of 18F-FDS, we developed a self-contained, solid-phase cartridge to rapidly (10 min) formulate ready-To-use 18F-FDS from commercially available 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG) at room temperature. In a hamster model, 18F-FDS PET/CT also differentiated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia from secondary Klebsiella pneumoniae pneumonia-A leading cause of complications in hospitalized patients with COVID-19. These data support 18F-FDS as an innovative and readily available, pathogen-specific PET technology with clinical applications.

Original languageEnglish (US)
Article numberabe9805
JournalScience translational medicine
Issue number589
StatePublished - Apr 14 2021

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Imaging Enterobacterales infections in patients using pathogen-specific positron emission tomography'. Together they form a unique fingerprint.

Cite this