Abstract
We recently developed a biosensor barcoding approach for highly multiplexed tracking of molecular activities in live cells. In this protocol, we detail the labeling of cells expressing different genetically encoded fluorescent biosensors with a pair of barcoding proteins and parallel imaging. Signals from cells with the same barcodes are then pooled together to obtain the dynamics of the corresponding biosensor activity. We describe the steps involved in cell barcoding, image acquisition, and analysis by deep learning models. For complete details on the use and execution of this protocol, please refer to Yang et al. (2021).
Original language | English (US) |
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Article number | 101611 |
Journal | STAR Protocols |
Volume | 3 |
Issue number | 3 |
DOIs | |
State | Published - Sep 16 2022 |
Keywords
- Cell biology
- Microscopy
- Molecular/Chemical probes
- Signal transduction
- Single cell
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
- General Neuroscience