@article{a4b026ce18b043c8b687ba6bd9f58b62,
title = "Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach",
abstract = "miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.",
keywords = "HT-1080, Mass spectrometry, MiRNA targets",
author = "Arivusudar Marimuthu and Huang, {Tai Chung} and Selvan, {Lakshmi Dhevi N.} and Santosh Renuse and Nirujogi, {Raja Sekhar} and Praveen Kumar and Pinto, {Sneha M.} and Sudha Rajagopalan and Akhilesh Pandey and Harsha, {H. C.} and Aditi Chatterjee",
note = "Funding Information: We thank the Department of Biotechnology (DBT) of the Government of India for research support to the Institute of Bioinformatics, Bangalore. Santosh Renuse is a recipient of Junior Research fellowship award from the University Grants Commission (UGC) of the Government of India. Sneha M. Pinto and Nirujogi Raja Sekhar are recipients of Senior Research fellowship award from the Council of Scientific and Industrial Research (CSIR) of the Government of India. Harsha Gowda is a Wellcome Trust/DBT India Alliance Early Career Fellow. Publisher Copyright: {\textcopyright} 2014 The Authors. Published by Elsevier B.V.",
year = "2014",
month = may,
day = "2",
doi = "10.1016/j.euprot.2014.04.006",
language = "English (US)",
volume = "4",
pages = "10--17",
journal = "EuPA Open Proteomics",
issn = "2212-9685",
publisher = "Elsevier BV",
}