Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season

Deena R. Blumenkrantz; Thomas Mehoke; Kathryn Shaw-Saliba; Harrison Powell; Nicholas Wohlgemuth; Hsuan Liu; Elizabeth MacIas; Jared Evans; Mitra Lewis; Rebecca Medina; Justin Hardick; Lauren M. Sauer; Andrea Dugas; Anna Duval; Andrew P. Lane; Charlotte Gaydos; Richard Rothman; Peter Thielen; Andrew Pekosz

doi:10.1093/ve/veab047

Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season

Deena R. Blumenkrantz, Thomas Mehoke, Kathryn Shaw-Saliba, Harrison Powell, Nicholas Wohlgemuth, Hsuan Liu, Elizabeth MacIas, Jared Evans, Mitra Lewis, Rebecca Medina, Justin Hardick, Lauren M. Sauer, Andrea Dugas, Anna Duval, Andrew P. Lane, Charlotte Gaydos, Richard Rothman, Peter Thielen, Andrew Pekosz

Research output: Contribution to journal › Article › peer-review

Abstract

The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.

Original language	English (US)
Article number	veab047
Journal	Virus Evolution
Volume	7
Issue number	1
DOIs	https://doi.org/10.1093/ve/veab047
State	Published - Jan 1 2021

Keywords

H3N2
NA
PB1-F2
antigenic drift
human
influenza
symptoms

ASJC Scopus subject areas

Microbiology
Virology

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10.1093/ve/veab047

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Blumenkrantz, D. R., Mehoke, T., Shaw-Saliba, K., Powell, H., Wohlgemuth, N., Liu, H., MacIas, E., Evans, J., Lewis, M., Medina, R., Hardick, J., Sauer, L. M., Dugas, A., Duval, A., Lane, A. P., Gaydos, C., Rothman, R., Thielen, P., & Pekosz, A. (2021). Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season. Virus Evolution, 7(1), Article veab047. https://doi.org/10.1093/ve/veab047

Blumenkrantz, DR, Mehoke, T, Shaw-Saliba, K, Powell, H, Wohlgemuth, N, Liu, H, MacIas, E, Evans, J, Lewis, M, Medina, R, Hardick, J, Sauer, LM, Dugas, A, Duval, A, Lane, AP , Gaydos, C , Rothman, R, Thielen, P & Pekosz, A 2021, 'Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season', Virus Evolution, vol. 7, no. 1, veab047. https://doi.org/10.1093/ve/veab047

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abstract = "The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.",

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AU - Powell, Harrison

AU - Wohlgemuth, Nicholas

AU - Liu, Hsuan

AU - MacIas, Elizabeth

AU - Evans, Jared

AU - Lewis, Mitra

AU - Medina, Rebecca

AU - Hardick, Justin

AU - Sauer, Lauren M.

AU - Dugas, Andrea

AU - Duval, Anna

AU - Lane, Andrew P.

AU - Gaydos, Charlotte

AU - Rothman, Richard

AU - Thielen, Peter

AU - Pekosz, Andrew

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AB - The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.

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Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season

Abstract

Keywords

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