Abstract
AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.
Original language | English (US) |
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Pages (from-to) | 366-371 |
Number of pages | 6 |
Journal | Acta Pharmacologica Sinica |
Volume | 25 |
Issue number | 3 |
State | Published - Mar 2004 |
Externally published | Yes |
Keywords
- Amino acid transport systems
- Glutamates
- Receptors
- Testis
- Uptake
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)