Identification of genes differentially expressed by hypoxia in hepatocellular carcinoma cells

Moon Kyoung Bae, Yoo Wook Kwon, Myoung Sook Kim, Soo Kyung Bae, Myung Ho Bae, You Mie Lee, Yung Jin Kim, Kyu Won Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


In order to identify genes differentially expressed under hypoxia (1% O2, 5% CO2, balance N2), we performed mRNA differential display analysis using total RNA extracted from hypoxic and normoxic HepG2, human hepatocellular carcinoma (HCC) cells. Of the differentially expressed genes by hypoxia, some of cDNA fragments were cloned and sequenced. The expression patterns of these clones by hypoxia were confirmed by Northern blot analysis and the quantitative RT-PCR. Down-regulated genes by hypoxia have homology to cDNA sequences encoding cytochrome oxidase subunit II and ADP/ATP translocase, respectively. Up-regulated gene by hypoxia was identified as Homo sapiens oscillin. Moreover, novel genes induced by hypoxia represent partial sequences of cDNAs that have not been reported or functionally identified. Up- or down-regulated expression of these genes in response to hypoxia may contribute to human hepatocarcinogenesis.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Feb 4 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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