Identification of extracellular vesicle-borne periostin as a feature of muscle-invasive bladder cancer

Christopher R. Silvers, Yu Ru Liu, Chia Hao Wu, Hiroshi Miyamoto, Edward M. Messing, Yi Fen Lee

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Muscle-invasive bladder cancer (MIBC) is an aggressive malignancy with high mortality, and heterogeneity in MIBC results in variable clinical outcomes, posing challenges for clinical management. Extracellular vesicles (EVs) derived from MIBC have been shown to promote cancer progression. EVs derived from bladder cell lines were subjected to proteomic analysis, and periostin was chosen for further characterization due to its stage-specific gene expression profile. Knockdown of periostin by RNA interference reduces invasiveness in vitro and produces a rounder morphology. Importantly, treating low grade BC cells with periostin-rich EVs promotes cell aggressiveness and activates ERK oncogenic signals, and periostin suppression reverses these effects. These data suggest that MIBC might transfer periostin in an EV-mediated paracrine manner to promote the disease. To determine the potential of periostin as a bladder cancer indicator, patient urinary EVs were examined and found to have markedly higher levels of periostin than controls. In addition, immunohistochemical staining of a bladder cancer tissue microarray revealed that the presence of periostin in MIBC cells is correlated with worse prognosis. In conclusion, periostin is a component of bladder cancer cells associated with poor clinical outcome, and EVs can transfer oncogenic molecules such as periostin to affect the tumor environment and promote cancer progression.

Original languageEnglish (US)
Pages (from-to)23335-23345
Number of pages11
Issue number17
StatePublished - Apr 26 2016


  • Exosome
  • Extracellular vesicle
  • Muscle-invasive bladder cancer and biomarker
  • Periostin

ASJC Scopus subject areas

  • Oncology


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