Identification of an MHC class I-restricted autoantigen in type 1 diabetes by screening an organ-specific cDNA library

F. Susan Wong, Jaana Karttunen, Caroline Dumont, Li Wen, Irene Visintin, Ingrid M. Pilip, Nilabh Shastri, Eric G. Pamer, Charles A. Janeway

Research output: Contribution to journalArticlepeer-review

370 Scopus citations

Abstract

Type 1 diabetes is an autoimmune disease in which the insulin-producing pancreatic β cells are destroyed at an early age by an immune process that involves both CD4 and CD8 T lymphocytes. The identification of autoantigens in diabetes is very important for the design of antigen-specific immunotherapy. By screening a pancreatic islet cDNA library, we have identified the autoantigen recognized by highly pathogenic CD8 T cells in the non-obese diabetic mouse, one of the best animal models for human diabetes. This is the first identification, to our knowledge, of a CD8 T-cell epitope in an autoimmune disease. The peptide recognized by the cells is in the same region of the insulin B chain as the epitope recognized by previously isolated pathogenic CD4 T cells. This has very important implications for the potential use of insulin in preventative therapy.

Original languageEnglish (US)
Pages (from-to)1026-1031
Number of pages6
JournalNature medicine
Volume5
Issue number9
DOIs
StatePublished - Sep 1999
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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