Abstract
p53 exerts important physiological functions in cell-cycle control, gene regulation, cell differentiation, apoptosis and tumor suppression by interacting with many cellular proteins. Using the yeast two-hybrid system, we screened a HeLa cDNA library and identified a novel gene encoding a p53-binding protein (p53BP3). The full-length cDNA of p53BP3 was isolated from a HeLaλgt10 cDNA library. This predicted protein was composed of 815 amino acids. Sequence analysis indicated that p53BP3 contained two bipartite nuclear localization signals and was confirmed to be a nuclear protein. FISH mapping results showed that this novel gene was located at human chromosome 12, region p11.2-p12.1. Northern blot analysis suggested that p53BP3 was broadly expressed in human tissues. A further study showed that p53BP3 had a homologue in mouse. (C) 1999 Elsevier Science B.V. All rights reserved.
Original language | English (US) |
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Pages (from-to) | 93-101 |
Number of pages | 9 |
Journal | Gene |
Volume | 235 |
Issue number | 1-2 |
DOIs | |
State | Published - Jul 22 1999 |
Externally published | Yes |
Keywords
- Chromosome 12p11.2-p12.1
- FISH
- Leucine zipper protein
- Yeast two-hybrid system
- p53
- p53-binding-protein
ASJC Scopus subject areas
- Genetics